Efficient Optimization of Gluconobacter oxydans Based on Protein Scaffold-Trimeric CutA to Enhance the Chemical Structure Stability of Enzymes for the Direct Production of 2-Keto-L-gulonic Acid
Joint Authors
Gao, Lili
Liu, Yuefeng
Zhang, Xiaoyu
Zhang, Hongsheng
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
2-Keto-L-gulonic acid (2-KLG), the direct precursor of vitamin C, is produced by a two-step fermentation route from D-sorbitol in industry.
However, this route is a complicated mix-culture system which involves three bacteria.
Thus, replacement of the conventional two-step fermentation process with a one-step process could be revolutionary in vitamin C industry.
The one-step fermentation of 2-keto-L-gulonic acid (2-KLG) has been achieved in our previous study; 32.4 g/L of 2-KLG production was obtained by the one-step strain G.
oxydans/pGUC-tufB-sdh-GGGGS-sndh after 168 h.
In this study, L-sorbose dehydrogenase (SDH) and L-sorbosone dehydrogenase (SNDH) were expressed in G.
oxydans after the codon optimization.
Furthermore, the trimeric protein CutA was used to improve the chemical structure stability of SDH and SNDH.
The recombinant strain G.
oxydans/pGUC-tufB-SH3-sdh-GGGGS-sndh-tufB-SH3lig-(GGGGS)2-cutA produced 40.3 g/L of 2-KLG after 168 h.
In addition, the expression levels of the cofactor PQQ were enhanced to further improve 2-KLG production.
With the stepwise metabolic engineering of G.
oxydans, the final 2-KLG production was improved to 42.6 g/L.
The efficient one-step production of 2-KLG was achieved, and the final one-step industrial-scale production of 2-KLG is drawing near.
American Psychological Association (APA)
Gao, Lili& Liu, Yuefeng& Zhang, Xiaoyu& Zhang, Hongsheng. 2020. Efficient Optimization of Gluconobacter oxydans Based on Protein Scaffold-Trimeric CutA to Enhance the Chemical Structure Stability of Enzymes for the Direct Production of 2-Keto-L-gulonic Acid. Journal of Chemistry،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1181889
Modern Language Association (MLA)
Gao, Lili…[et al.]. Efficient Optimization of Gluconobacter oxydans Based on Protein Scaffold-Trimeric CutA to Enhance the Chemical Structure Stability of Enzymes for the Direct Production of 2-Keto-L-gulonic Acid. Journal of Chemistry No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1181889
American Medical Association (AMA)
Gao, Lili& Liu, Yuefeng& Zhang, Xiaoyu& Zhang, Hongsheng. Efficient Optimization of Gluconobacter oxydans Based on Protein Scaffold-Trimeric CutA to Enhance the Chemical Structure Stability of Enzymes for the Direct Production of 2-Keto-L-gulonic Acid. Journal of Chemistry. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1181889
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1181889