Efficient Optimization of Gluconobacter oxydans Based on Protein Scaffold-Trimeric CutA to Enhance the Chemical Structure Stability of Enzymes for the Direct Production of 2-Keto-L-gulonic Acid

Joint Authors

Gao, Lili
Liu, Yuefeng
Zhang, Xiaoyu
Zhang, Hongsheng

Source

Journal of Chemistry

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-08-04

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Chemistry

Abstract EN

2-Keto-L-gulonic acid (2-KLG), the direct precursor of vitamin C, is produced by a two-step fermentation route from D-sorbitol in industry.

However, this route is a complicated mix-culture system which involves three bacteria.

Thus, replacement of the conventional two-step fermentation process with a one-step process could be revolutionary in vitamin C industry.

The one-step fermentation of 2-keto-L-gulonic acid (2-KLG) has been achieved in our previous study; 32.4 g/L of 2-KLG production was obtained by the one-step strain G.

oxydans/pGUC-tufB-sdh-GGGGS-sndh after 168 h.

In this study, L-sorbose dehydrogenase (SDH) and L-sorbosone dehydrogenase (SNDH) were expressed in G.

oxydans after the codon optimization.

Furthermore, the trimeric protein CutA was used to improve the chemical structure stability of SDH and SNDH.

The recombinant strain G.

oxydans/pGUC-tufB-SH3-sdh-GGGGS-sndh-tufB-SH3lig-(GGGGS)2-cutA produced 40.3 g/L of 2-KLG after 168 h.

In addition, the expression levels of the cofactor PQQ were enhanced to further improve 2-KLG production.

With the stepwise metabolic engineering of G.

oxydans, the final 2-KLG production was improved to 42.6 g/L.

The efficient one-step production of 2-KLG was achieved, and the final one-step industrial-scale production of 2-KLG is drawing near.

American Psychological Association (APA)

Gao, Lili& Liu, Yuefeng& Zhang, Xiaoyu& Zhang, Hongsheng. 2020. Efficient Optimization of Gluconobacter oxydans Based on Protein Scaffold-Trimeric CutA to Enhance the Chemical Structure Stability of Enzymes for the Direct Production of 2-Keto-L-gulonic Acid. Journal of Chemistry،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1181889

Modern Language Association (MLA)

Gao, Lili…[et al.]. Efficient Optimization of Gluconobacter oxydans Based on Protein Scaffold-Trimeric CutA to Enhance the Chemical Structure Stability of Enzymes for the Direct Production of 2-Keto-L-gulonic Acid. Journal of Chemistry No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1181889

American Medical Association (AMA)

Gao, Lili& Liu, Yuefeng& Zhang, Xiaoyu& Zhang, Hongsheng. Efficient Optimization of Gluconobacter oxydans Based on Protein Scaffold-Trimeric CutA to Enhance the Chemical Structure Stability of Enzymes for the Direct Production of 2-Keto-L-gulonic Acid. Journal of Chemistry. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1181889

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1181889