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Structural and Chemical Characterization of Silica Spheres before and after Modification by Silanization for Trypsin Immobilization
المؤلفون المشاركون
Silva, Luciano Paulino
Barbosa, Eduardo F.
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-07-13
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
In the last decades, silica particles of a variety of sizes and shapes have been characterized and chemically modified for several applications, from chromatographic separation to dental supplies.
The present study proposes the use of aminopropyl triethoxysilane (APTS) silanized silica particles to immobilize the proteolytic enzyme trypsin for the development of a bioreactor.
The major advantage of the process is that it enables the polypeptides hydrolysis interruption simply by removing the silica particles from the reaction bottle.
Silanized silica surfaces showed significant morphological changes at micro- and nanoscale level.
Chemical characterization showed changes in elemental composition, chemical environment, and thermal degradation.
Their application as supports for trypsin immobilization showed high immobilization efficiency at reduced immobilization times, combined with more acidic conditions.
Indirect immobilization quantification by reversed-phase ultrafast high performance liquid chromatography proved to be a suitable approach due to its high linearity and sensitivity.
Immobilized trypsin activities on nonmodified and silanized silica showed promising features (e.g., selective hydrolysis) for applications in proteins/peptides primary structure elucidation for proteomics.
Silanized silica system produced some preferential targeting peptides, probably due to the hydrophobicity of the nanoenvironment conditioned by silanization.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Barbosa, Eduardo F.& Silva, Luciano Paulino. 2017. Structural and Chemical Characterization of Silica Spheres before and after Modification by Silanization for Trypsin Immobilization. Journal of Nanomaterials،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1182699
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Barbosa, Eduardo F.& Silva, Luciano Paulino. Structural and Chemical Characterization of Silica Spheres before and after Modification by Silanization for Trypsin Immobilization. Journal of Nanomaterials No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1182699
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Barbosa, Eduardo F.& Silva, Luciano Paulino. Structural and Chemical Characterization of Silica Spheres before and after Modification by Silanization for Trypsin Immobilization. Journal of Nanomaterials. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1182699
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1182699
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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