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Structural and Chemical Characterization of Silica Spheres before and after Modification by Silanization for Trypsin Immobilization
Joint Authors
Silva, Luciano Paulino
Barbosa, Eduardo F.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In the last decades, silica particles of a variety of sizes and shapes have been characterized and chemically modified for several applications, from chromatographic separation to dental supplies.
The present study proposes the use of aminopropyl triethoxysilane (APTS) silanized silica particles to immobilize the proteolytic enzyme trypsin for the development of a bioreactor.
The major advantage of the process is that it enables the polypeptides hydrolysis interruption simply by removing the silica particles from the reaction bottle.
Silanized silica surfaces showed significant morphological changes at micro- and nanoscale level.
Chemical characterization showed changes in elemental composition, chemical environment, and thermal degradation.
Their application as supports for trypsin immobilization showed high immobilization efficiency at reduced immobilization times, combined with more acidic conditions.
Indirect immobilization quantification by reversed-phase ultrafast high performance liquid chromatography proved to be a suitable approach due to its high linearity and sensitivity.
Immobilized trypsin activities on nonmodified and silanized silica showed promising features (e.g., selective hydrolysis) for applications in proteins/peptides primary structure elucidation for proteomics.
Silanized silica system produced some preferential targeting peptides, probably due to the hydrophobicity of the nanoenvironment conditioned by silanization.
American Psychological Association (APA)
Barbosa, Eduardo F.& Silva, Luciano Paulino. 2017. Structural and Chemical Characterization of Silica Spheres before and after Modification by Silanization for Trypsin Immobilization. Journal of Nanomaterials،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1182699
Modern Language Association (MLA)
Barbosa, Eduardo F.& Silva, Luciano Paulino. Structural and Chemical Characterization of Silica Spheres before and after Modification by Silanization for Trypsin Immobilization. Journal of Nanomaterials No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1182699
American Medical Association (AMA)
Barbosa, Eduardo F.& Silva, Luciano Paulino. Structural and Chemical Characterization of Silica Spheres before and after Modification by Silanization for Trypsin Immobilization. Journal of Nanomaterials. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1182699
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182699