Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy
المؤلفون المشاركون
Xie, Minqiang
Zhang, Juan
Weng, Huanhuan
Miao, Xiangwan
Li, Quanming
Wang, Siqi
Xie, Huifen
Liu, Tao
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-12-12
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
A novel folate (FA) receptor-targeted superparamagnetic Fe3O4 nanoparticles (SPIONs) codelivering cisplatin (CDDP) and tissue factor pathway inhibitor-2 (TFPI-2) plasmid DNA (pDNA) was constructed.
The core shell nanocomposites (FA-PEG-PEI@SPION-CDDP-TFPI-2) were composed of superparamagnetic Fe3O4 core that binds CDDP and TFPI-2 shell that combines with folate-polyethylene glycol-polyethyleneimine (FA-PEG-PEI) via electrostatic interaction.
The shell containing FA-PEG-PEI and TFPI-2 plasmid was synthesized through amidation reaction and electrostatic adsorption and the core containing SPION-CDDP was modified by aldehyde sodium alginate.
Proton nuclear magnetic resonance and Fourier transform infrared spectra on FA-PEG-PEI polymers showed characteristic peaks of various metabolites in corresponding frequency.
Transmission electron microscopy image of FA-PEG-PEI@SPION-CDDP-TFPI-2 nanoparticles demonstrated a near-monodisperse spherical morphology, while dynamic light scattering studies indicated an intensity-average diameter of 149.5 nm.
Zeta potential was 14.89 ± 1.83 mv and the final concentration of loaded CDDP was 100 ug/ml.
Gel electrophoresis data showed that the nanocomposite would protect TFPI-2 pDNA from being digested by DNases.
Compared with CNE-2 cells, the good targetability and better gene transfection efficiency (57.9%) were detected by Prussian blue iron stain and fluorescence analysis in HNE-1 cells in vitro.
The results suggested the potential application of FA-PEG-PEI@SPION-CDDP-TFPI-2 as a multifunctional anticancer nanomedicine on targeting therapy for FR positive NPC.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Juan& Weng, Huanhuan& Miao, Xiangwan& Li, Quanming& Wang, Siqi& Xie, Huifen…[et al.]. 2017. Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy. Journal of Nanomaterials،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1182843
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Juan…[et al.]. Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy. Journal of Nanomaterials No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1182843
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Juan& Weng, Huanhuan& Miao, Xiangwan& Li, Quanming& Wang, Siqi& Xie, Huifen…[et al.]. Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy. Journal of Nanomaterials. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1182843
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1182843
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر