Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy
Joint Authors
Xie, Minqiang
Zhang, Juan
Weng, Huanhuan
Miao, Xiangwan
Li, Quanming
Wang, Siqi
Xie, Huifen
Liu, Tao
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A novel folate (FA) receptor-targeted superparamagnetic Fe3O4 nanoparticles (SPIONs) codelivering cisplatin (CDDP) and tissue factor pathway inhibitor-2 (TFPI-2) plasmid DNA (pDNA) was constructed.
The core shell nanocomposites (FA-PEG-PEI@SPION-CDDP-TFPI-2) were composed of superparamagnetic Fe3O4 core that binds CDDP and TFPI-2 shell that combines with folate-polyethylene glycol-polyethyleneimine (FA-PEG-PEI) via electrostatic interaction.
The shell containing FA-PEG-PEI and TFPI-2 plasmid was synthesized through amidation reaction and electrostatic adsorption and the core containing SPION-CDDP was modified by aldehyde sodium alginate.
Proton nuclear magnetic resonance and Fourier transform infrared spectra on FA-PEG-PEI polymers showed characteristic peaks of various metabolites in corresponding frequency.
Transmission electron microscopy image of FA-PEG-PEI@SPION-CDDP-TFPI-2 nanoparticles demonstrated a near-monodisperse spherical morphology, while dynamic light scattering studies indicated an intensity-average diameter of 149.5 nm.
Zeta potential was 14.89 ± 1.83 mv and the final concentration of loaded CDDP was 100 ug/ml.
Gel electrophoresis data showed that the nanocomposite would protect TFPI-2 pDNA from being digested by DNases.
Compared with CNE-2 cells, the good targetability and better gene transfection efficiency (57.9%) were detected by Prussian blue iron stain and fluorescence analysis in HNE-1 cells in vitro.
The results suggested the potential application of FA-PEG-PEI@SPION-CDDP-TFPI-2 as a multifunctional anticancer nanomedicine on targeting therapy for FR positive NPC.
American Psychological Association (APA)
Zhang, Juan& Weng, Huanhuan& Miao, Xiangwan& Li, Quanming& Wang, Siqi& Xie, Huifen…[et al.]. 2017. Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy. Journal of Nanomaterials،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1182843
Modern Language Association (MLA)
Zhang, Juan…[et al.]. Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy. Journal of Nanomaterials No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1182843
American Medical Association (AMA)
Zhang, Juan& Weng, Huanhuan& Miao, Xiangwan& Li, Quanming& Wang, Siqi& Xie, Huifen…[et al.]. Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy. Journal of Nanomaterials. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1182843
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182843