Rapid Detection and Identification of miRNAs by Surface-Enhanced Raman Spectroscopy Using Hollow Au Nanoflowers Substrates
المؤلفون المشاركون
Cao, Xiaowei
Bao, Min
Shan, Yibo
Li, Wei
Shi, Hongcan
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-10-19
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
MicroRNAs (miRNAs) are recognized as regulators of gene expression during the biological processes of cells as well as biomarkers of many diseases.
Development of rapid and sensitive miRNA profiling methods is crucial for evaluating the pattern of miRNA expression related to normal and diseased states.
This work presents a novel hollow Au nanoflowers (HAuNFs) substrate for rapid detection and identification of miRNAs by surface-enhanced Raman scattering (SERS) spectroscopy.
We synthesized the HAuNFs by a seed-mediated growth approach.
Then, HAuNFs substrates were fabricated by depositing HAuNFs onto the surfaces of (3-aminopropyl)triethoxysilane- (APTES-) functionalized ITO glass.
The result demonstrated that HAuNFs substrates had very good reproducibility, homogeneous SERS activity, and high SERS effect.
The substrates enabled us to successfully obtain the SERS spectra of miR-10a-5p, miR-125a-5p, and miR-196a-5p.
The difference spectra among the three kinds of miRNAs were studied to better interpret the spectral differences and identify miRNA expression patterns with high accuracy.
The principal component analysis (PCA) of the SERS spectra was used to distinguish among the three kinds of miRNAs.
Considering its time efficiency, being label-free, and its sensitivity, the SERS based on HAuNFs substrates is very promising for miRNA research and plays an important role in early disease detection and prevention.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Cao, Xiaowei& Bao, Min& Shan, Yibo& Li, Wei& Shi, Hongcan. 2017. Rapid Detection and Identification of miRNAs by Surface-Enhanced Raman Spectroscopy Using Hollow Au Nanoflowers Substrates. Journal of Nanomaterials،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1182926
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Cao, Xiaowei…[et al.]. Rapid Detection and Identification of miRNAs by Surface-Enhanced Raman Spectroscopy Using Hollow Au Nanoflowers Substrates. Journal of Nanomaterials No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1182926
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Cao, Xiaowei& Bao, Min& Shan, Yibo& Li, Wei& Shi, Hongcan. Rapid Detection and Identification of miRNAs by Surface-Enhanced Raman Spectroscopy Using Hollow Au Nanoflowers Substrates. Journal of Nanomaterials. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1182926
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1182926
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر