Rapid Detection and Identification of miRNAs by Surface-Enhanced Raman Spectroscopy Using Hollow Au Nanoflowers Substrates
Joint Authors
Cao, Xiaowei
Bao, Min
Shan, Yibo
Li, Wei
Shi, Hongcan
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
MicroRNAs (miRNAs) are recognized as regulators of gene expression during the biological processes of cells as well as biomarkers of many diseases.
Development of rapid and sensitive miRNA profiling methods is crucial for evaluating the pattern of miRNA expression related to normal and diseased states.
This work presents a novel hollow Au nanoflowers (HAuNFs) substrate for rapid detection and identification of miRNAs by surface-enhanced Raman scattering (SERS) spectroscopy.
We synthesized the HAuNFs by a seed-mediated growth approach.
Then, HAuNFs substrates were fabricated by depositing HAuNFs onto the surfaces of (3-aminopropyl)triethoxysilane- (APTES-) functionalized ITO glass.
The result demonstrated that HAuNFs substrates had very good reproducibility, homogeneous SERS activity, and high SERS effect.
The substrates enabled us to successfully obtain the SERS spectra of miR-10a-5p, miR-125a-5p, and miR-196a-5p.
The difference spectra among the three kinds of miRNAs were studied to better interpret the spectral differences and identify miRNA expression patterns with high accuracy.
The principal component analysis (PCA) of the SERS spectra was used to distinguish among the three kinds of miRNAs.
Considering its time efficiency, being label-free, and its sensitivity, the SERS based on HAuNFs substrates is very promising for miRNA research and plays an important role in early disease detection and prevention.
American Psychological Association (APA)
Cao, Xiaowei& Bao, Min& Shan, Yibo& Li, Wei& Shi, Hongcan. 2017. Rapid Detection and Identification of miRNAs by Surface-Enhanced Raman Spectroscopy Using Hollow Au Nanoflowers Substrates. Journal of Nanomaterials،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1182926
Modern Language Association (MLA)
Cao, Xiaowei…[et al.]. Rapid Detection and Identification of miRNAs by Surface-Enhanced Raman Spectroscopy Using Hollow Au Nanoflowers Substrates. Journal of Nanomaterials No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1182926
American Medical Association (AMA)
Cao, Xiaowei& Bao, Min& Shan, Yibo& Li, Wei& Shi, Hongcan. Rapid Detection and Identification of miRNAs by Surface-Enhanced Raman Spectroscopy Using Hollow Au Nanoflowers Substrates. Journal of Nanomaterials. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1182926
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182926