Detection of Carbapenem-Resistant Genes in Escherichia coli Isolated from Drinking Water in Khartoum, Sudan
المؤلفون المشاركون
Mahmoud, Neama Esmat
Altayb, Hisham N.
Gurashi, Reem Majzoub
المصدر
Journal of Environmental and Public Health
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-06-12
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Waterborne Escherichia coli are a major reservoir of antimicrobial resistance (AMR).
Carbapenem-resistance, especially when mediated by transferable carbapenemase-encoding genes, is spreading worldwide and causing dramatically limiting treatment options.
In our country, studies for the detection of carbapenem resistance in drinking water do not exist; therefore, this work was carried out to determine the prevalence of carbapenem-resistant genes “blaKPC, blaIMP, blaNDM, blaSPM, blaVIM, and blaOXA-48” among Escherichia coli isolated from drinking water in Khartoum, Sudan.
A total of forty-five E.
coli bacteria were isolated from different sources of drinking water.
Antimicrobial susceptibility testing was performed using imipenem (10 mg/disc), gentamicin (10 mg/disc), ceftriaxone (30 mg/disc), ciprofloxacin (5 mg/disc), chloramphenicol (30 mg/disc), and tetracycline (30 mg/disc).
“Sensitive” or “resistant” patterns of E.
coli were judged using antibiotic minimum inhibitory concentration (MIC).
Bacterial genomic DNA was extracted by the boiling method, and then multiplex polymerase chain reaction was performed to detect the carbapenemase genes (blaKPC, blaIMP, blaNDM, blaSPM, blaVIM, and blaOXA-48).
Multiplex PCR assays confirmed the presence of carbapenemase genes in 28% of all water isolates.
OXA-48 gene was the most predominant gene, detected in 15.5% of the isolates.
The blaKPC and blaSPM genes were also detected in 4.4% and 8.8% of the isolates, respectively.
However, the isolates were negative for blaNDM, blaVIM, and blaIMP genes.
The isolates showed a high rate of tetracycline resistance (97.7%), followed by gentamicin (57.7%), ciprofloxacin (46.6%), ceftriaxone (35.5%), and chloramphenicol (31.1%).
In conclusion, this study confirmed for the first time the presence of E.
coli carried carbapenem-resistant genes in the drinking water of Khartoum state, Sudan.
These isolates commonly carried OXA-48 (7/45), followed by SPM (4/45) and KPC (2/45).
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mahmoud, Neama Esmat& Altayb, Hisham N.& Gurashi, Reem Majzoub. 2020. Detection of Carbapenem-Resistant Genes in Escherichia coli Isolated from Drinking Water in Khartoum, Sudan. Journal of Environmental and Public Health،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1184224
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mahmoud, Neama Esmat…[et al.]. Detection of Carbapenem-Resistant Genes in Escherichia coli Isolated from Drinking Water in Khartoum, Sudan. Journal of Environmental and Public Health No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1184224
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mahmoud, Neama Esmat& Altayb, Hisham N.& Gurashi, Reem Majzoub. Detection of Carbapenem-Resistant Genes in Escherichia coli Isolated from Drinking Water in Khartoum, Sudan. Journal of Environmental and Public Health. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1184224
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1184224
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر