Detection of Carbapenem-Resistant Genes in Escherichia coli Isolated from Drinking Water in Khartoum, Sudan
Joint Authors
Mahmoud, Neama Esmat
Altayb, Hisham N.
Gurashi, Reem Majzoub
Source
Journal of Environmental and Public Health
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-12
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Waterborne Escherichia coli are a major reservoir of antimicrobial resistance (AMR).
Carbapenem-resistance, especially when mediated by transferable carbapenemase-encoding genes, is spreading worldwide and causing dramatically limiting treatment options.
In our country, studies for the detection of carbapenem resistance in drinking water do not exist; therefore, this work was carried out to determine the prevalence of carbapenem-resistant genes “blaKPC, blaIMP, blaNDM, blaSPM, blaVIM, and blaOXA-48” among Escherichia coli isolated from drinking water in Khartoum, Sudan.
A total of forty-five E.
coli bacteria were isolated from different sources of drinking water.
Antimicrobial susceptibility testing was performed using imipenem (10 mg/disc), gentamicin (10 mg/disc), ceftriaxone (30 mg/disc), ciprofloxacin (5 mg/disc), chloramphenicol (30 mg/disc), and tetracycline (30 mg/disc).
“Sensitive” or “resistant” patterns of E.
coli were judged using antibiotic minimum inhibitory concentration (MIC).
Bacterial genomic DNA was extracted by the boiling method, and then multiplex polymerase chain reaction was performed to detect the carbapenemase genes (blaKPC, blaIMP, blaNDM, blaSPM, blaVIM, and blaOXA-48).
Multiplex PCR assays confirmed the presence of carbapenemase genes in 28% of all water isolates.
OXA-48 gene was the most predominant gene, detected in 15.5% of the isolates.
The blaKPC and blaSPM genes were also detected in 4.4% and 8.8% of the isolates, respectively.
However, the isolates were negative for blaNDM, blaVIM, and blaIMP genes.
The isolates showed a high rate of tetracycline resistance (97.7%), followed by gentamicin (57.7%), ciprofloxacin (46.6%), ceftriaxone (35.5%), and chloramphenicol (31.1%).
In conclusion, this study confirmed for the first time the presence of E.
coli carried carbapenem-resistant genes in the drinking water of Khartoum state, Sudan.
These isolates commonly carried OXA-48 (7/45), followed by SPM (4/45) and KPC (2/45).
American Psychological Association (APA)
Mahmoud, Neama Esmat& Altayb, Hisham N.& Gurashi, Reem Majzoub. 2020. Detection of Carbapenem-Resistant Genes in Escherichia coli Isolated from Drinking Water in Khartoum, Sudan. Journal of Environmental and Public Health،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1184224
Modern Language Association (MLA)
Mahmoud, Neama Esmat…[et al.]. Detection of Carbapenem-Resistant Genes in Escherichia coli Isolated from Drinking Water in Khartoum, Sudan. Journal of Environmental and Public Health No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1184224
American Medical Association (AMA)
Mahmoud, Neama Esmat& Altayb, Hisham N.& Gurashi, Reem Majzoub. Detection of Carbapenem-Resistant Genes in Escherichia coli Isolated from Drinking Water in Khartoum, Sudan. Journal of Environmental and Public Health. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1184224
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1184224