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Quantitative Detection of Beef and Beef Meat Products Adulteration by the Addition of Duck Meat Using Micro Drop Digital Polymerase Chain Reaction
المؤلفون المشاركون
Chen, Chen
Chen, Jia
Zhang, Yan
Li, Yongbo
Wang, Zan
Li, Xian
Zhou, Wei
Zhang, Zhisheng
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-28
دولة النشر
مصر
عدد الصفحات
8
الملخص EN
A single-copy specific primer was designed based on beef and duck samples and through drop digital polymerase chain reaction (ddPCR) for the quantitative analysis.
Results revealed that the primers had no specific amplification with sheep, chicken, pork, or other species.
Both the relationships between meat weight and DNA weight and between DNA weight and DNA copy number (C) were nearly linear within the dynamic range.
To calculate the original meat weight from the DNA copy number, the DNA weight was used as the intermediate value to establish the following formulae: Mbeef = 0.058C − 1.86; Mduck = 0.0268C − 7.78.
To achieve a good quantitative analysis, all species used in the experiment were made of lean meat.
The accuracy of the method was verified by artificial adulteration of different proportions.
Testing of the commercial samples indicated that adulteration is present in the market.
The established digital PCR method provided an effective tool for monitoring the adulterated meat products and reducing the adulteration in the market.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Chen& Chen, Jia& Zhang, Yan& Li, Yongbo& Wang, Zan& Li, Xian…[et al.]. 2020. Quantitative Detection of Beef and Beef Meat Products Adulteration by the Addition of Duck Meat Using Micro Drop Digital Polymerase Chain Reaction. Journal of Food Quality،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1184612
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Chen…[et al.]. Quantitative Detection of Beef and Beef Meat Products Adulteration by the Addition of Duck Meat Using Micro Drop Digital Polymerase Chain Reaction. Journal of Food Quality No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1184612
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Chen& Chen, Jia& Zhang, Yan& Li, Yongbo& Wang, Zan& Li, Xian…[et al.]. Quantitative Detection of Beef and Beef Meat Products Adulteration by the Addition of Duck Meat Using Micro Drop Digital Polymerase Chain Reaction. Journal of Food Quality. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1184612
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1184612
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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