Quantitative Detection of Beef and Beef Meat Products Adulteration by the Addition of Duck Meat Using Micro Drop Digital Polymerase Chain Reaction
Joint Authors
Chen, Chen
Chen, Jia
Zhang, Yan
Li, Yongbo
Wang, Zan
Li, Xian
Zhou, Wei
Zhang, Zhisheng
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-28
Country of Publication
Egypt
No. of Pages
8
Abstract EN
A single-copy specific primer was designed based on beef and duck samples and through drop digital polymerase chain reaction (ddPCR) for the quantitative analysis.
Results revealed that the primers had no specific amplification with sheep, chicken, pork, or other species.
Both the relationships between meat weight and DNA weight and between DNA weight and DNA copy number (C) were nearly linear within the dynamic range.
To calculate the original meat weight from the DNA copy number, the DNA weight was used as the intermediate value to establish the following formulae: Mbeef = 0.058C − 1.86; Mduck = 0.0268C − 7.78.
To achieve a good quantitative analysis, all species used in the experiment were made of lean meat.
The accuracy of the method was verified by artificial adulteration of different proportions.
Testing of the commercial samples indicated that adulteration is present in the market.
The established digital PCR method provided an effective tool for monitoring the adulterated meat products and reducing the adulteration in the market.
American Psychological Association (APA)
Chen, Chen& Chen, Jia& Zhang, Yan& Li, Yongbo& Wang, Zan& Li, Xian…[et al.]. 2020. Quantitative Detection of Beef and Beef Meat Products Adulteration by the Addition of Duck Meat Using Micro Drop Digital Polymerase Chain Reaction. Journal of Food Quality،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1184612
Modern Language Association (MLA)
Chen, Chen…[et al.]. Quantitative Detection of Beef and Beef Meat Products Adulteration by the Addition of Duck Meat Using Micro Drop Digital Polymerase Chain Reaction. Journal of Food Quality No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1184612
American Medical Association (AMA)
Chen, Chen& Chen, Jia& Zhang, Yan& Li, Yongbo& Wang, Zan& Li, Xian…[et al.]. Quantitative Detection of Beef and Beef Meat Products Adulteration by the Addition of Duck Meat Using Micro Drop Digital Polymerase Chain Reaction. Journal of Food Quality. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1184612
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1184612