Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL
المؤلفون المشاركون
Zhang, Min
Yuan, Yaowu
Gao, Hao
Tan, Jianxin
Tian, Yiling
Liu, Jianhui
Ma, Aijin
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-10-22
دولة النشر
مصر
عدد الصفحات
11
الملخص EN
Agaricus bisporus lectin (ABL), which is one of the antinutritional factors in A.
bisporus, is an important allergen and harmful to human health.
Due to the shortcomings of the current detection methods, it is extremely urgent to establish a rapid and sensitive detection method for ABL in foods.
To isolate the ssDNA aptamer of ABL, 13 rounds of subtractive systematic evolution of ligands by exponential enrichment (SELEX) selection were carried out.
As a result, six candidate aptamers were selected and further examined for their binding affinity and specificity by enzyme-linked aptamer method.
One aptamer (seq-41) against ABL with a high affinity and specificity was isolated and demonstrated to be the optimal aptamer whose dissociation constant reaches the nanomolar level, Kd = 31.17 ± 0.1070 nM.
Based on seq-41, an aptamer-AuNPs colorimetric method was established to detect ABL with a linear range of 0.08∼1.70 μg/mL and the detection limit is 0.062 μg/mL.
This study provides a novel aptamer-AuNPs colorimetric method with high sensitivity and specificity for detection of ABL and a novel strategy for development of detection method of fungal or plant allergens.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gao, Hao& Tian, Yiling& Zhang, Min& Liu, Jianhui& Yuan, Yaowu& Tan, Jianxin…[et al.]. 2020. Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL. Journal of Food Quality،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1184843
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gao, Hao…[et al.]. Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL. Journal of Food Quality No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1184843
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gao, Hao& Tian, Yiling& Zhang, Min& Liu, Jianhui& Yuan, Yaowu& Tan, Jianxin…[et al.]. Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL. Journal of Food Quality. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1184843
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1184843
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر