Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL
Joint Authors
Zhang, Min
Yuan, Yaowu
Gao, Hao
Tan, Jianxin
Tian, Yiling
Liu, Jianhui
Ma, Aijin
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-22
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Agaricus bisporus lectin (ABL), which is one of the antinutritional factors in A.
bisporus, is an important allergen and harmful to human health.
Due to the shortcomings of the current detection methods, it is extremely urgent to establish a rapid and sensitive detection method for ABL in foods.
To isolate the ssDNA aptamer of ABL, 13 rounds of subtractive systematic evolution of ligands by exponential enrichment (SELEX) selection were carried out.
As a result, six candidate aptamers were selected and further examined for their binding affinity and specificity by enzyme-linked aptamer method.
One aptamer (seq-41) against ABL with a high affinity and specificity was isolated and demonstrated to be the optimal aptamer whose dissociation constant reaches the nanomolar level, Kd = 31.17 ± 0.1070 nM.
Based on seq-41, an aptamer-AuNPs colorimetric method was established to detect ABL with a linear range of 0.08∼1.70 μg/mL and the detection limit is 0.062 μg/mL.
This study provides a novel aptamer-AuNPs colorimetric method with high sensitivity and specificity for detection of ABL and a novel strategy for development of detection method of fungal or plant allergens.
American Psychological Association (APA)
Gao, Hao& Tian, Yiling& Zhang, Min& Liu, Jianhui& Yuan, Yaowu& Tan, Jianxin…[et al.]. 2020. Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL. Journal of Food Quality،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1184843
Modern Language Association (MLA)
Gao, Hao…[et al.]. Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL. Journal of Food Quality No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1184843
American Medical Association (AMA)
Gao, Hao& Tian, Yiling& Zhang, Min& Liu, Jianhui& Yuan, Yaowu& Tan, Jianxin…[et al.]. Selection, Identification, and Application of Aptamers against Agaricus bisporus Lectin to Establish an Aptamer-AuNPs Colorimetric Method for Detection of ABL. Journal of Food Quality. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1184843
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1184843