Inhibition of Rho Kinase Induces Antioxidative Molecules and Suppresses Reactive Oxidative Species in Trabecular Meshwork Cells
المؤلفون المشاركون
Fujimoto, Tomokazu
Inoue, Toshihiro
Ohira, Saori
Awai-Kasaoka, Nanako
Kameda, Takanori
Inoue-Mochita, Miyuki
Tanihara, Hidenobu
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-23، 23ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-07-19
دولة النشر
مصر
عدد الصفحات
23
التخصصات الرئيسية
الملخص EN
Purpose.
To investigate the effect of rho kinase inhibitors on oxidative stress in trabecular meshwork (TM) cells.
Methods.
TM cells were isolated from the eyes of cynomolgus monkeys.
Y-27632 and menadione were used to inhibit rho kinase and induce production of reactive oxygen species (ROS), respectively.
The cynomolgus monkey array and 12,613 probes were used in DNA microarray analysis, and the affected genes were categorized using gene ontology analysis.
The mRNA levels of the target genes were confirmed by real-time RT-PCR.
Intracellular oxidative stress was detected using a fluorescent reagent sensitive to ROS.
Cell viability was assessed by the WST-8 assay.
Results.
Gene ontology analysis revealed upregulation of genes involved in antioxidant activity, and upregulation of catalase was confirmed by real-time RT-PCR after 30 min treatment with Y-27632.
Production of ROS was increased by menadione, and the effect was partly suppressed by pretreatment with Y-27632.
At a lower dose of menadione, Y-27632 stimulated TM cells and significantly increased their viability following menadione treatment compared to control cells.
Conclusion.
Using microarray analysis, Y-27632 was shown to upregulate antioxidative genes including catalase and partially reduce ROS production and cell death by oxidative stress caused by menadione.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Fujimoto, Tomokazu& Inoue, Toshihiro& Ohira, Saori& Awai-Kasaoka, Nanako& Kameda, Takanori& Inoue-Mochita, Miyuki…[et al.]. 2017. Inhibition of Rho Kinase Induces Antioxidative Molecules and Suppresses Reactive Oxidative Species in Trabecular Meshwork Cells. Journal of Ophthalmology،Vol. 2017, no. 2017, pp.1-23.
https://search.emarefa.net/detail/BIM-1185362
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Fujimoto, Tomokazu…[et al.]. Inhibition of Rho Kinase Induces Antioxidative Molecules and Suppresses Reactive Oxidative Species in Trabecular Meshwork Cells. Journal of Ophthalmology No. 2017 (2017), pp.1-23.
https://search.emarefa.net/detail/BIM-1185362
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Fujimoto, Tomokazu& Inoue, Toshihiro& Ohira, Saori& Awai-Kasaoka, Nanako& Kameda, Takanori& Inoue-Mochita, Miyuki…[et al.]. Inhibition of Rho Kinase Induces Antioxidative Molecules and Suppresses Reactive Oxidative Species in Trabecular Meshwork Cells. Journal of Ophthalmology. 2017. Vol. 2017, no. 2017, pp.1-23.
https://search.emarefa.net/detail/BIM-1185362
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1185362
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر