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Inhibition of Rho Kinase Induces Antioxidative Molecules and Suppresses Reactive Oxidative Species in Trabecular Meshwork Cells
Joint Authors
Fujimoto, Tomokazu
Inoue, Toshihiro
Ohira, Saori
Awai-Kasaoka, Nanako
Kameda, Takanori
Inoue-Mochita, Miyuki
Tanihara, Hidenobu
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-23, 23 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-19
Country of Publication
Egypt
No. of Pages
23
Main Subjects
Abstract EN
Purpose.
To investigate the effect of rho kinase inhibitors on oxidative stress in trabecular meshwork (TM) cells.
Methods.
TM cells were isolated from the eyes of cynomolgus monkeys.
Y-27632 and menadione were used to inhibit rho kinase and induce production of reactive oxygen species (ROS), respectively.
The cynomolgus monkey array and 12,613 probes were used in DNA microarray analysis, and the affected genes were categorized using gene ontology analysis.
The mRNA levels of the target genes were confirmed by real-time RT-PCR.
Intracellular oxidative stress was detected using a fluorescent reagent sensitive to ROS.
Cell viability was assessed by the WST-8 assay.
Results.
Gene ontology analysis revealed upregulation of genes involved in antioxidant activity, and upregulation of catalase was confirmed by real-time RT-PCR after 30 min treatment with Y-27632.
Production of ROS was increased by menadione, and the effect was partly suppressed by pretreatment with Y-27632.
At a lower dose of menadione, Y-27632 stimulated TM cells and significantly increased their viability following menadione treatment compared to control cells.
Conclusion.
Using microarray analysis, Y-27632 was shown to upregulate antioxidative genes including catalase and partially reduce ROS production and cell death by oxidative stress caused by menadione.
American Psychological Association (APA)
Fujimoto, Tomokazu& Inoue, Toshihiro& Ohira, Saori& Awai-Kasaoka, Nanako& Kameda, Takanori& Inoue-Mochita, Miyuki…[et al.]. 2017. Inhibition of Rho Kinase Induces Antioxidative Molecules and Suppresses Reactive Oxidative Species in Trabecular Meshwork Cells. Journal of Ophthalmology،Vol. 2017, no. 2017, pp.1-23.
https://search.emarefa.net/detail/BIM-1185362
Modern Language Association (MLA)
Fujimoto, Tomokazu…[et al.]. Inhibition of Rho Kinase Induces Antioxidative Molecules and Suppresses Reactive Oxidative Species in Trabecular Meshwork Cells. Journal of Ophthalmology No. 2017 (2017), pp.1-23.
https://search.emarefa.net/detail/BIM-1185362
American Medical Association (AMA)
Fujimoto, Tomokazu& Inoue, Toshihiro& Ohira, Saori& Awai-Kasaoka, Nanako& Kameda, Takanori& Inoue-Mochita, Miyuki…[et al.]. Inhibition of Rho Kinase Induces Antioxidative Molecules and Suppresses Reactive Oxidative Species in Trabecular Meshwork Cells. Journal of Ophthalmology. 2017. Vol. 2017, no. 2017, pp.1-23.
https://search.emarefa.net/detail/BIM-1185362
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1185362