Construction of Two mCherry Plasmids (pXG-mCherry)‎ for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis

المؤلفون المشاركون

Vacas, Andrés
Sugden, Conor
Velasco-Rodriguez, Óscar
Algarabel-Olona, Miriam
Peña-Guerrero, José
Larrea, Esther
Fernández-Rubio, Celia
Nguewa, Paul A.

المصدر

Journal of Parasitology Research

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-11، 11ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-02-13

دولة النشر

مصر

عدد الصفحات

11

التخصصات الرئيسية

علم الحيوان
الأمراض

الملخص EN

Leishmania is the causative agent of leishmaniasis, a neglected tropical disease that affects more than 12 million people around the world.

Current treatments are toxic and poorly effective due to the acquisition of resistance within Leishmania populations.

Thus, the pursuit for new antileishmanial drugs is a priority.

The available methods for drug screening based on colorimetric assays using vital dyes are time-consuming.

Currently, the use of fluorescent reporter proteins is replacing the use of viability indicator dyes.

We have constructed two plasmids expressing the red fluorescent protein mCherry with multiple cloning sites (MCS), adequate for N- and C-terminal fusion protein constructs.

Our results also show that the improved pXG-mCherry plasmid can be employed for drug screening in vitro.

The use of the red fluorescent protein, mCherry, is an easier tool for numerous assays, not only to test pharmacological compounds, but also to determine the subcellular localization of proteins.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Vacas, Andrés& Sugden, Conor& Velasco-Rodriguez, Óscar& Algarabel-Olona, Miriam& Peña-Guerrero, José& Larrea, Esther…[et al.]. 2017. Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis. Journal of Parasitology Research،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1186176

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Vacas, Andrés…[et al.]. Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis. Journal of Parasitology Research No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1186176

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Vacas, Andrés& Sugden, Conor& Velasco-Rodriguez, Óscar& Algarabel-Olona, Miriam& Peña-Guerrero, José& Larrea, Esther…[et al.]. Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis. Journal of Parasitology Research. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1186176

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1186176