Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis
Joint Authors
Vacas, Andrés
Sugden, Conor
Velasco-Rodriguez, Óscar
Algarabel-Olona, Miriam
Peña-Guerrero, José
Larrea, Esther
Fernández-Rubio, Celia
Nguewa, Paul A.
Source
Journal of Parasitology Research
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Leishmania is the causative agent of leishmaniasis, a neglected tropical disease that affects more than 12 million people around the world.
Current treatments are toxic and poorly effective due to the acquisition of resistance within Leishmania populations.
Thus, the pursuit for new antileishmanial drugs is a priority.
The available methods for drug screening based on colorimetric assays using vital dyes are time-consuming.
Currently, the use of fluorescent reporter proteins is replacing the use of viability indicator dyes.
We have constructed two plasmids expressing the red fluorescent protein mCherry with multiple cloning sites (MCS), adequate for N- and C-terminal fusion protein constructs.
Our results also show that the improved pXG-mCherry plasmid can be employed for drug screening in vitro.
The use of the red fluorescent protein, mCherry, is an easier tool for numerous assays, not only to test pharmacological compounds, but also to determine the subcellular localization of proteins.
American Psychological Association (APA)
Vacas, Andrés& Sugden, Conor& Velasco-Rodriguez, Óscar& Algarabel-Olona, Miriam& Peña-Guerrero, José& Larrea, Esther…[et al.]. 2017. Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis. Journal of Parasitology Research،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1186176
Modern Language Association (MLA)
Vacas, Andrés…[et al.]. Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis. Journal of Parasitology Research No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1186176
American Medical Association (AMA)
Vacas, Andrés& Sugden, Conor& Velasco-Rodriguez, Óscar& Algarabel-Olona, Miriam& Peña-Guerrero, José& Larrea, Esther…[et al.]. Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis. Journal of Parasitology Research. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1186176
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1186176