Construction of Two mCherry Plasmids (pXG-mCherry)‎ for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis

Joint Authors

Vacas, Andrés
Sugden, Conor
Velasco-Rodriguez, Óscar
Algarabel-Olona, Miriam
Peña-Guerrero, José
Larrea, Esther
Fernández-Rubio, Celia
Nguewa, Paul A.

Source

Journal of Parasitology Research

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-02-13

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Zoology
Diseases

Abstract EN

Leishmania is the causative agent of leishmaniasis, a neglected tropical disease that affects more than 12 million people around the world.

Current treatments are toxic and poorly effective due to the acquisition of resistance within Leishmania populations.

Thus, the pursuit for new antileishmanial drugs is a priority.

The available methods for drug screening based on colorimetric assays using vital dyes are time-consuming.

Currently, the use of fluorescent reporter proteins is replacing the use of viability indicator dyes.

We have constructed two plasmids expressing the red fluorescent protein mCherry with multiple cloning sites (MCS), adequate for N- and C-terminal fusion protein constructs.

Our results also show that the improved pXG-mCherry plasmid can be employed for drug screening in vitro.

The use of the red fluorescent protein, mCherry, is an easier tool for numerous assays, not only to test pharmacological compounds, but also to determine the subcellular localization of proteins.

American Psychological Association (APA)

Vacas, Andrés& Sugden, Conor& Velasco-Rodriguez, Óscar& Algarabel-Olona, Miriam& Peña-Guerrero, José& Larrea, Esther…[et al.]. 2017. Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis. Journal of Parasitology Research،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1186176

Modern Language Association (MLA)

Vacas, Andrés…[et al.]. Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis. Journal of Parasitology Research No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1186176

American Medical Association (AMA)

Vacas, Andrés& Sugden, Conor& Velasco-Rodriguez, Óscar& Algarabel-Olona, Miriam& Peña-Guerrero, José& Larrea, Esther…[et al.]. Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis. Journal of Parasitology Research. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1186176

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1186176