Selecting PCR for the Diagnosis of Intestinal Parasitosis: Choice of Targets, Evaluation of In-House Assays, and Comparison with Commercial Kits

المؤلفون المشاركون

Hartmeyer, G. N.
Hoegh, S. V.
Skov, M. N.
Dessau, R. B.
Kemp, M.

المصدر

Journal of Parasitology Research

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-6، 6ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-08-30

دولة النشر

مصر

عدد الصفحات

6

التخصصات الرئيسية

علم الحيوان
الأمراض

الملخص EN

Microscopy of stool samples is a labour-intensive and inaccurate technique for detection of intestinal parasites causing diarrhoea and replacement by PCR is attractive.

Almost all cases of diarrhoea induced by parasites over a nine-year period in our laboratory were due to Giardia lamblia, Cryptosporidium species, or Entamoeba histolytica detected by microscopy.

We evaluated and selected in-house singleplex real-time PCR (RT-PCR) assays for these pathogens in 99 stool samples from patients suspected of having intestinal parasitosis tested by microscopy.

The strategy included a genus-specific PCR assay for C.

parvum and C.

hominis, with subsequent identification by a PCR that distinguishes between the two species.

G.

lamblia was detected in five and C.

parvum in one out of 68 microscopy-negative samples.

The performance of the in-house RT-PCR assays was compared to three commercially available multiplex test (MT-PCR) kit systems in 81 stool samples, collected in 28 microscopy-positive and 27 microscopy-negative samples from individuals suspected of intestinal parasitosis and in 26 samples from individuals without suspicion of parasitic infection.

The in-house assays detected parasites in more samples from patients suspected of having parasitosis than did any of the kits.

We conclude that commercial kits are targeting relevant parasites, but their performance may vary.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Hartmeyer, G. N.& Hoegh, S. V.& Skov, M. N.& Dessau, R. B.& Kemp, M.. 2017. Selecting PCR for the Diagnosis of Intestinal Parasitosis: Choice of Targets, Evaluation of In-House Assays, and Comparison with Commercial Kits. Journal of Parasitology Research،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1186223

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Hartmeyer, G. N.…[et al.]. Selecting PCR for the Diagnosis of Intestinal Parasitosis: Choice of Targets, Evaluation of In-House Assays, and Comparison with Commercial Kits. Journal of Parasitology Research No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1186223

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Hartmeyer, G. N.& Hoegh, S. V.& Skov, M. N.& Dessau, R. B.& Kemp, M.. Selecting PCR for the Diagnosis of Intestinal Parasitosis: Choice of Targets, Evaluation of In-House Assays, and Comparison with Commercial Kits. Journal of Parasitology Research. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1186223

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1186223