Selecting PCR for the Diagnosis of Intestinal Parasitosis: Choice of Targets, Evaluation of In-House Assays, and Comparison with Commercial Kits
Joint Authors
Hartmeyer, G. N.
Hoegh, S. V.
Skov, M. N.
Dessau, R. B.
Kemp, M.
Source
Journal of Parasitology Research
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-30
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Microscopy of stool samples is a labour-intensive and inaccurate technique for detection of intestinal parasites causing diarrhoea and replacement by PCR is attractive.
Almost all cases of diarrhoea induced by parasites over a nine-year period in our laboratory were due to Giardia lamblia, Cryptosporidium species, or Entamoeba histolytica detected by microscopy.
We evaluated and selected in-house singleplex real-time PCR (RT-PCR) assays for these pathogens in 99 stool samples from patients suspected of having intestinal parasitosis tested by microscopy.
The strategy included a genus-specific PCR assay for C.
parvum and C.
hominis, with subsequent identification by a PCR that distinguishes between the two species.
G.
lamblia was detected in five and C.
parvum in one out of 68 microscopy-negative samples.
The performance of the in-house RT-PCR assays was compared to three commercially available multiplex test (MT-PCR) kit systems in 81 stool samples, collected in 28 microscopy-positive and 27 microscopy-negative samples from individuals suspected of intestinal parasitosis and in 26 samples from individuals without suspicion of parasitic infection.
The in-house assays detected parasites in more samples from patients suspected of having parasitosis than did any of the kits.
We conclude that commercial kits are targeting relevant parasites, but their performance may vary.
American Psychological Association (APA)
Hartmeyer, G. N.& Hoegh, S. V.& Skov, M. N.& Dessau, R. B.& Kemp, M.. 2017. Selecting PCR for the Diagnosis of Intestinal Parasitosis: Choice of Targets, Evaluation of In-House Assays, and Comparison with Commercial Kits. Journal of Parasitology Research،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1186223
Modern Language Association (MLA)
Hartmeyer, G. N.…[et al.]. Selecting PCR for the Diagnosis of Intestinal Parasitosis: Choice of Targets, Evaluation of In-House Assays, and Comparison with Commercial Kits. Journal of Parasitology Research No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1186223
American Medical Association (AMA)
Hartmeyer, G. N.& Hoegh, S. V.& Skov, M. N.& Dessau, R. B.& Kemp, M.. Selecting PCR for the Diagnosis of Intestinal Parasitosis: Choice of Targets, Evaluation of In-House Assays, and Comparison with Commercial Kits. Journal of Parasitology Research. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1186223
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1186223