Notch Signaling Pathway Is Involved in bFGF-Induced Corneal Lymphangiogenesis and Hemangiogenesis
المؤلفون المشاركون
Xie, Fang
Zhang, Xue
Luo, Wenting
Ge, Hongyan
Sun, Dawei
Liu, Ping
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-08-20
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Background.
Notch/Dll4 involvement in cornea neovascularization (CRNV) and lymphangiogenesis is unclear.
This study aimed to explore the role of notch signaling in basic fibroblast growth factor- (bFGF-) induced corneal lymphangiogenesis and hemangiogenesis.
Methods.
Corneal stroma of C57BL/6 mice was implanted with bFGF- or phosphate-buffered saline- (PBS-) soaked pellets.
Corneal lymphangiogenesis and neovascularization were evaluated by immunofluorescence.
Vascular endothelial growth factor-A (VEGF-A), Delta-like ligand 4 (Dll4), and Notch1 mRNA and protein expression were examined on days 1, 3, 7, and 14 by real-time polymerase chain reaction and western blot.
Corneal cells were treated with ranibizumab, dexamethasone, and γ-secretase inhibitor (GSI).
Microspheres were used to evaluate corneal hemangiogenesis in vivo.
Results.
Corneal hemangiogenesis reached its peak on day 7 after bFGF implantation, and corneal lymphangiogenesis was significantly higher on day 7 and 14, compared with PBS.
mRNA and protein expression of VEGF-A, Dll4, and Notch1 were higher in bFGF-induced animal models compared with controls.
Corneal hemangiogenesis and lymphangiogenesis decreased after 7 days of ranibizumab or dexamethasone treatment.
After adding GSI for 24 h in bFGF-induced cells, the expression of Notch1 and Dll4 were downregulated compared with that in the control group whereas the expression level of VEGF-A was upregulated.
Fluorescent particle number was higher in the GSI group.
Ranibizumab and dexamethasone decreased the fluorescence signal.
Conclusion.
The notch signaling pathway plays a role in regulating VEGF expression, affecting corneal lymphangiogenesis and hemangiogenesis in mice.
The molecular imaging probe technique can visualize the changes in the VEGF-A expression level of corneal limbus hemangiogenesis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xie, Fang& Zhang, Xue& Luo, Wenting& Ge, Hongyan& Sun, Dawei& Liu, Ping. 2019. Notch Signaling Pathway Is Involved in bFGF-Induced Corneal Lymphangiogenesis and Hemangiogenesis. Journal of Ophthalmology،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1186488
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xie, Fang…[et al.]. Notch Signaling Pathway Is Involved in bFGF-Induced Corneal Lymphangiogenesis and Hemangiogenesis. Journal of Ophthalmology No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1186488
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xie, Fang& Zhang, Xue& Luo, Wenting& Ge, Hongyan& Sun, Dawei& Liu, Ping. Notch Signaling Pathway Is Involved in bFGF-Induced Corneal Lymphangiogenesis and Hemangiogenesis. Journal of Ophthalmology. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1186488
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1186488
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر