Notch Signaling Pathway Is Involved in bFGF-Induced Corneal Lymphangiogenesis and Hemangiogenesis
Joint Authors
Xie, Fang
Zhang, Xue
Luo, Wenting
Ge, Hongyan
Sun, Dawei
Liu, Ping
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-08-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background.
Notch/Dll4 involvement in cornea neovascularization (CRNV) and lymphangiogenesis is unclear.
This study aimed to explore the role of notch signaling in basic fibroblast growth factor- (bFGF-) induced corneal lymphangiogenesis and hemangiogenesis.
Methods.
Corneal stroma of C57BL/6 mice was implanted with bFGF- or phosphate-buffered saline- (PBS-) soaked pellets.
Corneal lymphangiogenesis and neovascularization were evaluated by immunofluorescence.
Vascular endothelial growth factor-A (VEGF-A), Delta-like ligand 4 (Dll4), and Notch1 mRNA and protein expression were examined on days 1, 3, 7, and 14 by real-time polymerase chain reaction and western blot.
Corneal cells were treated with ranibizumab, dexamethasone, and γ-secretase inhibitor (GSI).
Microspheres were used to evaluate corneal hemangiogenesis in vivo.
Results.
Corneal hemangiogenesis reached its peak on day 7 after bFGF implantation, and corneal lymphangiogenesis was significantly higher on day 7 and 14, compared with PBS.
mRNA and protein expression of VEGF-A, Dll4, and Notch1 were higher in bFGF-induced animal models compared with controls.
Corneal hemangiogenesis and lymphangiogenesis decreased after 7 days of ranibizumab or dexamethasone treatment.
After adding GSI for 24 h in bFGF-induced cells, the expression of Notch1 and Dll4 were downregulated compared with that in the control group whereas the expression level of VEGF-A was upregulated.
Fluorescent particle number was higher in the GSI group.
Ranibizumab and dexamethasone decreased the fluorescence signal.
Conclusion.
The notch signaling pathway plays a role in regulating VEGF expression, affecting corneal lymphangiogenesis and hemangiogenesis in mice.
The molecular imaging probe technique can visualize the changes in the VEGF-A expression level of corneal limbus hemangiogenesis.
American Psychological Association (APA)
Xie, Fang& Zhang, Xue& Luo, Wenting& Ge, Hongyan& Sun, Dawei& Liu, Ping. 2019. Notch Signaling Pathway Is Involved in bFGF-Induced Corneal Lymphangiogenesis and Hemangiogenesis. Journal of Ophthalmology،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1186488
Modern Language Association (MLA)
Xie, Fang…[et al.]. Notch Signaling Pathway Is Involved in bFGF-Induced Corneal Lymphangiogenesis and Hemangiogenesis. Journal of Ophthalmology No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1186488
American Medical Association (AMA)
Xie, Fang& Zhang, Xue& Luo, Wenting& Ge, Hongyan& Sun, Dawei& Liu, Ping. Notch Signaling Pathway Is Involved in bFGF-Induced Corneal Lymphangiogenesis and Hemangiogenesis. Journal of Ophthalmology. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1186488
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1186488