Preferential Expression of B7-H6 in Glioma Stem-Like Cells Enhances Tumor Cell Proliferation via the c-MycRNMT Axis
المؤلفون المشاركون
Sun, Jing
Guo, Yundi
Chen, Hanqing
Dong, Jun
Bao, Qinghua
Fu, Fengqing
Zhang, XueGuang
المصدر
Journal of Immunology Research
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-04-06
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
B7 homologue 6 (B7-H6), a newly identified member of the B7 costimulatory molecule family, is not only a crucial regulator of NK cell-mediated immune responses through binding to NKp30 but also has clinical implications due to its abnormal expression in human cancers.
Here, we show that B7-H6 expression is abnormally upregulated in glioma tissue and that B7-H6 is coexpressed with stem cell marker Sox2.
Intriguingly, B7-H6 was rarely detected on the surface of glioma cell lines but was abundantly expressed in glioma stem-like cells (GSLCs) that were derived from the glioma cell lines in vitro.
Surprisingly, B7-H6 was the only one that was preferentially expressed in the GSLCs among the B7 family members.
Functionally, knockdown of B7-H6 in GSLCs by siRNAs led to the inhibition of cell proliferation, with decrease in the expression of the oncogene Myc as well as inactivation of PI3K/Akt and ERK/MAPK signaling pathways.
Moreover, we determined that three genes CBL (Casitas B-Lineage Lymphoma Proto-Oncogene), CCNT1 (Cyclin T1), and RNMT (RNA guanine-7 methyltransferase) were coexpressed with B7-H6 and c-myc in glioma tissue samples from the TCGA database and found, however that only RNMT expression was inhibited by the knockdown of B7-H6 expression in the GSLCs, suggesting the involvement of RNMT in the B7-H6/c-myc axis.
Extending this to 293T cells, we observed that knocking out of B7-H6 with CRISPR-Cas9 system also suppressed cell proliferation.
Thus, our findings suggest B7-H6 as a potential molecule for glioma stem cell targeted immunotherapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Hanqing& Guo, Yundi& Sun, Jing& Dong, Jun& Bao, Qinghua& Zhang, XueGuang…[et al.]. 2020. Preferential Expression of B7-H6 in Glioma Stem-Like Cells Enhances Tumor Cell Proliferation via the c-MycRNMT Axis. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1186997
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Hanqing…[et al.]. Preferential Expression of B7-H6 in Glioma Stem-Like Cells Enhances Tumor Cell Proliferation via the c-MycRNMT Axis. Journal of Immunology Research No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1186997
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Hanqing& Guo, Yundi& Sun, Jing& Dong, Jun& Bao, Qinghua& Zhang, XueGuang…[et al.]. Preferential Expression of B7-H6 in Glioma Stem-Like Cells Enhances Tumor Cell Proliferation via the c-MycRNMT Axis. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1186997
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1186997
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر