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Preferential Expression of B7-H6 in Glioma Stem-Like Cells Enhances Tumor Cell Proliferation via the c-MycRNMT Axis
Joint Authors
Sun, Jing
Guo, Yundi
Chen, Hanqing
Dong, Jun
Bao, Qinghua
Fu, Fengqing
Zhang, XueGuang
Source
Journal of Immunology Research
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-06
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
B7 homologue 6 (B7-H6), a newly identified member of the B7 costimulatory molecule family, is not only a crucial regulator of NK cell-mediated immune responses through binding to NKp30 but also has clinical implications due to its abnormal expression in human cancers.
Here, we show that B7-H6 expression is abnormally upregulated in glioma tissue and that B7-H6 is coexpressed with stem cell marker Sox2.
Intriguingly, B7-H6 was rarely detected on the surface of glioma cell lines but was abundantly expressed in glioma stem-like cells (GSLCs) that were derived from the glioma cell lines in vitro.
Surprisingly, B7-H6 was the only one that was preferentially expressed in the GSLCs among the B7 family members.
Functionally, knockdown of B7-H6 in GSLCs by siRNAs led to the inhibition of cell proliferation, with decrease in the expression of the oncogene Myc as well as inactivation of PI3K/Akt and ERK/MAPK signaling pathways.
Moreover, we determined that three genes CBL (Casitas B-Lineage Lymphoma Proto-Oncogene), CCNT1 (Cyclin T1), and RNMT (RNA guanine-7 methyltransferase) were coexpressed with B7-H6 and c-myc in glioma tissue samples from the TCGA database and found, however that only RNMT expression was inhibited by the knockdown of B7-H6 expression in the GSLCs, suggesting the involvement of RNMT in the B7-H6/c-myc axis.
Extending this to 293T cells, we observed that knocking out of B7-H6 with CRISPR-Cas9 system also suppressed cell proliferation.
Thus, our findings suggest B7-H6 as a potential molecule for glioma stem cell targeted immunotherapy.
American Psychological Association (APA)
Chen, Hanqing& Guo, Yundi& Sun, Jing& Dong, Jun& Bao, Qinghua& Zhang, XueGuang…[et al.]. 2020. Preferential Expression of B7-H6 in Glioma Stem-Like Cells Enhances Tumor Cell Proliferation via the c-MycRNMT Axis. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1186997
Modern Language Association (MLA)
Chen, Hanqing…[et al.]. Preferential Expression of B7-H6 in Glioma Stem-Like Cells Enhances Tumor Cell Proliferation via the c-MycRNMT Axis. Journal of Immunology Research No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1186997
American Medical Association (AMA)
Chen, Hanqing& Guo, Yundi& Sun, Jing& Dong, Jun& Bao, Qinghua& Zhang, XueGuang…[et al.]. Preferential Expression of B7-H6 in Glioma Stem-Like Cells Enhances Tumor Cell Proliferation via the c-MycRNMT Axis. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1186997
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1186997