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Attenuated Subcomponent Vaccine Design Targeting the SARS-CoV-2 Nucleocapsid Phosphoprotein RNA Binding Domain: In Silico Analysis
المؤلفون المشاركون
Chukwudozie, Onyeka S.
Chukwuanukwu, Rebecca C.
Iroanya, Onyekachi O.
Eze, Daniel M.
Duru, Vincent C.
Dele-Alimi, Temiloluwa O.
Kehinde, Busuyi D.
Bankole, Taiwo T.
Obi, Perpetua C.
Okinedo, Elizabeth U.
المصدر
Journal of Immunology Research
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-09-17
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
The novel coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has previously never been identified with humans, thereby creating devastation in public health.
The need for an effective vaccine to curb this pandemic cannot be overemphasized.
In view of this, we designed a subcomponent antigenic peptide vaccine targeting the N-terminal (NT) and C-terminal (CT) RNA binding domains of the nucleocapsid protein that aid in viral replication.
Promising antigenic B cell and T cell epitopes were predicted using computational pipelines.
The peptides “RIRGGDGKMKDL” and “AFGRRGPEQTQGNFG” were the B cell linear epitopes with good antigenic index and nonallergenic property.
Two CD8+ and Three CD4+ T cell epitopes were also selected considering their safe immunogenic profiling such as allergenicity, antigen level conservancy, antigenicity, peptide toxicity, and putative restrictions to a number of MHC-I and MHC-II alleles.
With these selected epitopes, a nonallergenic chimeric peptide vaccine incapable of inducing a type II hypersensitivity reaction was constructed.
The molecular interaction between the Toll-like receptor-5 (TLR5) which was triggered by the vaccine was analyzed by molecular docking and scrutinized using dynamics simulation.
Finally, in silico cloning was performed to ensure the expression and translation efficiency of the vaccine, utilizing the pET-28a vector.
This research, therefore, provides a guide for experimental investigation and validation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chukwudozie, Onyeka S.& Chukwuanukwu, Rebecca C.& Iroanya, Onyekachi O.& Eze, Daniel M.& Duru, Vincent C.& Dele-Alimi, Temiloluwa O.…[et al.]. 2020. Attenuated Subcomponent Vaccine Design Targeting the SARS-CoV-2 Nucleocapsid Phosphoprotein RNA Binding Domain: In Silico Analysis. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1187080
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chukwudozie, Onyeka S.…[et al.]. Attenuated Subcomponent Vaccine Design Targeting the SARS-CoV-2 Nucleocapsid Phosphoprotein RNA Binding Domain: In Silico Analysis. Journal of Immunology Research No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1187080
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chukwudozie, Onyeka S.& Chukwuanukwu, Rebecca C.& Iroanya, Onyekachi O.& Eze, Daniel M.& Duru, Vincent C.& Dele-Alimi, Temiloluwa O.…[et al.]. Attenuated Subcomponent Vaccine Design Targeting the SARS-CoV-2 Nucleocapsid Phosphoprotein RNA Binding Domain: In Silico Analysis. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1187080
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1187080
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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