Attenuated Subcomponent Vaccine Design Targeting the SARS-CoV-2 Nucleocapsid Phosphoprotein RNA Binding Domain: In Silico Analysis
Joint Authors
Chukwudozie, Onyeka S.
Chukwuanukwu, Rebecca C.
Iroanya, Onyekachi O.
Eze, Daniel M.
Duru, Vincent C.
Dele-Alimi, Temiloluwa O.
Kehinde, Busuyi D.
Bankole, Taiwo T.
Obi, Perpetua C.
Okinedo, Elizabeth U.
Source
Journal of Immunology Research
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-17
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
The novel coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has previously never been identified with humans, thereby creating devastation in public health.
The need for an effective vaccine to curb this pandemic cannot be overemphasized.
In view of this, we designed a subcomponent antigenic peptide vaccine targeting the N-terminal (NT) and C-terminal (CT) RNA binding domains of the nucleocapsid protein that aid in viral replication.
Promising antigenic B cell and T cell epitopes were predicted using computational pipelines.
The peptides “RIRGGDGKMKDL” and “AFGRRGPEQTQGNFG” were the B cell linear epitopes with good antigenic index and nonallergenic property.
Two CD8+ and Three CD4+ T cell epitopes were also selected considering their safe immunogenic profiling such as allergenicity, antigen level conservancy, antigenicity, peptide toxicity, and putative restrictions to a number of MHC-I and MHC-II alleles.
With these selected epitopes, a nonallergenic chimeric peptide vaccine incapable of inducing a type II hypersensitivity reaction was constructed.
The molecular interaction between the Toll-like receptor-5 (TLR5) which was triggered by the vaccine was analyzed by molecular docking and scrutinized using dynamics simulation.
Finally, in silico cloning was performed to ensure the expression and translation efficiency of the vaccine, utilizing the pET-28a vector.
This research, therefore, provides a guide for experimental investigation and validation.
American Psychological Association (APA)
Chukwudozie, Onyeka S.& Chukwuanukwu, Rebecca C.& Iroanya, Onyekachi O.& Eze, Daniel M.& Duru, Vincent C.& Dele-Alimi, Temiloluwa O.…[et al.]. 2020. Attenuated Subcomponent Vaccine Design Targeting the SARS-CoV-2 Nucleocapsid Phosphoprotein RNA Binding Domain: In Silico Analysis. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1187080
Modern Language Association (MLA)
Chukwudozie, Onyeka S.…[et al.]. Attenuated Subcomponent Vaccine Design Targeting the SARS-CoV-2 Nucleocapsid Phosphoprotein RNA Binding Domain: In Silico Analysis. Journal of Immunology Research No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1187080
American Medical Association (AMA)
Chukwudozie, Onyeka S.& Chukwuanukwu, Rebecca C.& Iroanya, Onyekachi O.& Eze, Daniel M.& Duru, Vincent C.& Dele-Alimi, Temiloluwa O.…[et al.]. Attenuated Subcomponent Vaccine Design Targeting the SARS-CoV-2 Nucleocapsid Phosphoprotein RNA Binding Domain: In Silico Analysis. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1187080
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187080