Development of an Immunoassay for the Detection of Amyloid Beta 1-42 and Its Application in Urine Samples
المؤلفون المشاركون
Wongta, Anurak
Hongsibsong, Surat
Chantara, Somporn
Pattarawarapan, Mookda
Sapbamrer, Ratana
Sringarm, Korawan
Xu, Zhen-Lin
Wang, Hong
المصدر
Journal of Immunology Research
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-12-28
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Amyloid beta peptides (Aβ1-42) have been found to be associated with the cause of Alzheimer’s disease (AD) and dementia.
Currently, methods for detecting Aβ1-42 are complicated and expensive.
The present study is aimed at developing an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) to detect Aβ1-42 by using a polyclonal antibody from alpaca, an application used in urine samples.
The serum was collected from the alpaca after immunizing it with Aβ1-42 at 500 μg/injection 5 times.
The ic-ELISA was developed and showed a half-maximal inhibitory concentration (IC50) of 103.20 ng/ml.
The limit of detection (LOD) was 0.39 ng/100 μl.
The cross-reactivity was tested with Aβ1-40 and 8 synthesized peptides that had sequence similarities to parts of Aβ1-42.
The cross-reactivity of Aβ1-40 and peptide 1 (DAEFRHDSGYE) was 55% and 69.4%, respectively.
The ic-ELISA was applied to analyze Aβ1-42 in the urine and precipitated protein urine samples.
This method can be used for detecting a normal level of total soluble Aβ (approximately 1 ng in 5 mg of precipitated urine protein) and can be used for detecting the early stages of AD.
It is considered to be an easy and inexpensive method for monitoring and diagnosing AD.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wongta, Anurak& Hongsibsong, Surat& Chantara, Somporn& Pattarawarapan, Mookda& Sapbamrer, Ratana& Sringarm, Korawan…[et al.]. 2020. Development of an Immunoassay for the Detection of Amyloid Beta 1-42 and Its Application in Urine Samples. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1187610
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wongta, Anurak…[et al.]. Development of an Immunoassay for the Detection of Amyloid Beta 1-42 and Its Application in Urine Samples. Journal of Immunology Research No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1187610
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wongta, Anurak& Hongsibsong, Surat& Chantara, Somporn& Pattarawarapan, Mookda& Sapbamrer, Ratana& Sringarm, Korawan…[et al.]. Development of an Immunoassay for the Detection of Amyloid Beta 1-42 and Its Application in Urine Samples. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1187610
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1187610
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر