Development of an Immunoassay for the Detection of Amyloid Beta 1-42 and Its Application in Urine Samples

Joint Authors

Wongta, Anurak
Hongsibsong, Surat
Chantara, Somporn
Pattarawarapan, Mookda
Sapbamrer, Ratana
Sringarm, Korawan
Xu, Zhen-Lin
Wang, Hong

Source

Journal of Immunology Research

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-12-28

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Biology

Abstract EN

Amyloid beta peptides (Aβ1-42) have been found to be associated with the cause of Alzheimer’s disease (AD) and dementia.

Currently, methods for detecting Aβ1-42 are complicated and expensive.

The present study is aimed at developing an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) to detect Aβ1-42 by using a polyclonal antibody from alpaca, an application used in urine samples.

The serum was collected from the alpaca after immunizing it with Aβ1-42 at 500 μg/injection 5 times.

The ic-ELISA was developed and showed a half-maximal inhibitory concentration (IC50) of 103.20 ng/ml.

The limit of detection (LOD) was 0.39 ng/100 μl.

The cross-reactivity was tested with Aβ1-40 and 8 synthesized peptides that had sequence similarities to parts of Aβ1-42.

The cross-reactivity of Aβ1-40 and peptide 1 (DAEFRHDSGYE) was 55% and 69.4%, respectively.

The ic-ELISA was applied to analyze Aβ1-42 in the urine and precipitated protein urine samples.

This method can be used for detecting a normal level of total soluble Aβ (approximately 1 ng in 5 mg of precipitated urine protein) and can be used for detecting the early stages of AD.

It is considered to be an easy and inexpensive method for monitoring and diagnosing AD.

American Psychological Association (APA)

Wongta, Anurak& Hongsibsong, Surat& Chantara, Somporn& Pattarawarapan, Mookda& Sapbamrer, Ratana& Sringarm, Korawan…[et al.]. 2020. Development of an Immunoassay for the Detection of Amyloid Beta 1-42 and Its Application in Urine Samples. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1187610

Modern Language Association (MLA)

Wongta, Anurak…[et al.]. Development of an Immunoassay for the Detection of Amyloid Beta 1-42 and Its Application in Urine Samples. Journal of Immunology Research No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1187610

American Medical Association (AMA)

Wongta, Anurak& Hongsibsong, Surat& Chantara, Somporn& Pattarawarapan, Mookda& Sapbamrer, Ratana& Sringarm, Korawan…[et al.]. Development of an Immunoassay for the Detection of Amyloid Beta 1-42 and Its Application in Urine Samples. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1187610

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1187610