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SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-κB Inflammatory Signaling in Rat Mesangial Cells
المؤلفون المشاركون
Long, Yang
Liang, Yaling
Dong, Jianhua
Zhou, Luping
Jiang, Chunxia
Chen, Meijuan
Xu, Yong
Huang, Wei
Gao, Chenlin
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-09-05
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background.
Sumoylation is extensively involved in the regulation of NF-κB signaling.
PIASy, as a SUMO E3 ligase, has been proved to mediate sumoylation of IκB kinase γ (IKKγ) and contribute to the activation of NF-κB under genotoxic agent stimulation.
However, the association of PIASy and NF-κB signaling in the pathogenesis of diabetic nephropathy (DN) has not been defined.
Methods.
Rat glomerular mesangial cells (GMCs) were stimulated by high glucose; siRNA was constructed to silence the expression of PIASy; the expression of PIASy, SUMO isoforms (SUMO1, SUMO2/3), and NF-κB signaling components was analyzed by Western blot; the interaction between IKKγ and SUMO proteins was detected by coimmunoprecipitation; and the release of inflammatory cytokines MCP-1 and IL-6 was assayed by ELISA.
Results.
High glucose significantly upregulated the expression of PIASy, SUMO1, and SUMO2/3 in a dose- and time-dependent manner (P<0.05), induced the phosphorylation and sumoylation of IKKγ (P<0.05), and then triggered NF-κB signaling whereas MCP-1 and IL-6 were released from GMCs (P<0.05).
Moreover, these high glucose-induced effects were observably reversed by siRNA-mediated knockdown of PIASy (P<0.05).
Conclusion.
The SUMO E3 ligase PIASy mediates high glucose-induced activation of NF-κB inflammatory signaling, suggesting that PIASy may be a potential therapeutic target of DN.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Huang, Wei& Liang, Yaling& Dong, Jianhua& Zhou, Luping& Gao, Chenlin& Jiang, Chunxia…[et al.]. 2017. SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-κB Inflammatory Signaling in Rat Mesangial Cells. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1188110
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Huang, Wei…[et al.]. SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-κB Inflammatory Signaling in Rat Mesangial Cells. Mediators of Inflammation No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1188110
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Huang, Wei& Liang, Yaling& Dong, Jianhua& Zhou, Luping& Gao, Chenlin& Jiang, Chunxia…[et al.]. SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-κB Inflammatory Signaling in Rat Mesangial Cells. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1188110
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1188110
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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