SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-κB Inflammatory Signaling in Rat Mesangial Cells
Joint Authors
Long, Yang
Liang, Yaling
Dong, Jianhua
Zhou, Luping
Jiang, Chunxia
Chen, Meijuan
Xu, Yong
Huang, Wei
Gao, Chenlin
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Sumoylation is extensively involved in the regulation of NF-κB signaling.
PIASy, as a SUMO E3 ligase, has been proved to mediate sumoylation of IκB kinase γ (IKKγ) and contribute to the activation of NF-κB under genotoxic agent stimulation.
However, the association of PIASy and NF-κB signaling in the pathogenesis of diabetic nephropathy (DN) has not been defined.
Methods.
Rat glomerular mesangial cells (GMCs) were stimulated by high glucose; siRNA was constructed to silence the expression of PIASy; the expression of PIASy, SUMO isoforms (SUMO1, SUMO2/3), and NF-κB signaling components was analyzed by Western blot; the interaction between IKKγ and SUMO proteins was detected by coimmunoprecipitation; and the release of inflammatory cytokines MCP-1 and IL-6 was assayed by ELISA.
Results.
High glucose significantly upregulated the expression of PIASy, SUMO1, and SUMO2/3 in a dose- and time-dependent manner (P<0.05), induced the phosphorylation and sumoylation of IKKγ (P<0.05), and then triggered NF-κB signaling whereas MCP-1 and IL-6 were released from GMCs (P<0.05).
Moreover, these high glucose-induced effects were observably reversed by siRNA-mediated knockdown of PIASy (P<0.05).
Conclusion.
The SUMO E3 ligase PIASy mediates high glucose-induced activation of NF-κB inflammatory signaling, suggesting that PIASy may be a potential therapeutic target of DN.
American Psychological Association (APA)
Huang, Wei& Liang, Yaling& Dong, Jianhua& Zhou, Luping& Gao, Chenlin& Jiang, Chunxia…[et al.]. 2017. SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-κB Inflammatory Signaling in Rat Mesangial Cells. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1188110
Modern Language Association (MLA)
Huang, Wei…[et al.]. SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-κB Inflammatory Signaling in Rat Mesangial Cells. Mediators of Inflammation No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1188110
American Medical Association (AMA)
Huang, Wei& Liang, Yaling& Dong, Jianhua& Zhou, Luping& Gao, Chenlin& Jiang, Chunxia…[et al.]. SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-κB Inflammatory Signaling in Rat Mesangial Cells. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1188110
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188110