Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts

المؤلفون المشاركون

Surinlert, Piyaporn
Kongthong, Nitchamon
Watthanard, Mariam
Sae-lao, Thannicha
Sookbangnop, Piyawat
Pholpramool, Chumpol
Tipbunjong, Chittipong

المصدر

Journal of Toxicology

العدد

المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-11، 11ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2020-05-11

دولة النشر

مصر

عدد الصفحات

11

التخصصات الرئيسية

الطب البشري

الملخص EN

Contaminations of chemicals in foods and drinks are raising public concerns.

Among these, styrene, a monomer for plastic production, receives increasing interest due to its ability to leach from the packaging and contaminate in foods and drinks causing many health problems.

The present study was designed to investigate the effects of styrene monomer (STR) and its metabolite styrene oxide (STO) on C2C12 myoblast proliferation and differentiation.

Based on an MTT assay, both STR and STO showed no cytotoxic effect at 10–100 μM.

However, at 50–100 μM STO, but not STR, significantly inhibited cell proliferation.

The STO-treated cells were accumulated in S-phase of cell cycles as revealed by flow cytometry.

The antioxidant enzyme (catalase and superoxide dismutase) activities and the gene expressing these enzymes of the arrested cells were decreased and ultimately led to nuclear condensation and expression of apoptotic markers such as cleaved caspase-3 and-9, but not cleaved caspase-8.

In addition, STO significantly suppressed myogenic differentiation by decreasing both the number and size of differentiated myotubes.

Biochemical analysis showed attenuations of total protein synthesis and myosin heavy chain (MHC) protein expression.

In conclusion, a metabolite of styrene, STO, leached from plastic packaging of foods and beverages suppressed both myoblast proliferation and differentiation, which would affect skeletal muscle development and regeneration.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Surinlert, Piyaporn& Kongthong, Nitchamon& Watthanard, Mariam& Sae-lao, Thannicha& Sookbangnop, Piyawat& Pholpramool, Chumpol…[et al.]. 2020. Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts. Journal of Toxicology،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1190878

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Surinlert, Piyaporn…[et al.]. Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts. Journal of Toxicology No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1190878

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Surinlert, Piyaporn& Kongthong, Nitchamon& Watthanard, Mariam& Sae-lao, Thannicha& Sookbangnop, Piyawat& Pholpramool, Chumpol…[et al.]. Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts. Journal of Toxicology. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1190878

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1190878