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Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts
Joint Authors
Surinlert, Piyaporn
Kongthong, Nitchamon
Watthanard, Mariam
Sae-lao, Thannicha
Sookbangnop, Piyawat
Pholpramool, Chumpol
Tipbunjong, Chittipong
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-11
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Contaminations of chemicals in foods and drinks are raising public concerns.
Among these, styrene, a monomer for plastic production, receives increasing interest due to its ability to leach from the packaging and contaminate in foods and drinks causing many health problems.
The present study was designed to investigate the effects of styrene monomer (STR) and its metabolite styrene oxide (STO) on C2C12 myoblast proliferation and differentiation.
Based on an MTT assay, both STR and STO showed no cytotoxic effect at 10–100 μM.
However, at 50–100 μM STO, but not STR, significantly inhibited cell proliferation.
The STO-treated cells were accumulated in S-phase of cell cycles as revealed by flow cytometry.
The antioxidant enzyme (catalase and superoxide dismutase) activities and the gene expressing these enzymes of the arrested cells were decreased and ultimately led to nuclear condensation and expression of apoptotic markers such as cleaved caspase-3 and-9, but not cleaved caspase-8.
In addition, STO significantly suppressed myogenic differentiation by decreasing both the number and size of differentiated myotubes.
Biochemical analysis showed attenuations of total protein synthesis and myosin heavy chain (MHC) protein expression.
In conclusion, a metabolite of styrene, STO, leached from plastic packaging of foods and beverages suppressed both myoblast proliferation and differentiation, which would affect skeletal muscle development and regeneration.
American Psychological Association (APA)
Surinlert, Piyaporn& Kongthong, Nitchamon& Watthanard, Mariam& Sae-lao, Thannicha& Sookbangnop, Piyawat& Pholpramool, Chumpol…[et al.]. 2020. Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts. Journal of Toxicology،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1190878
Modern Language Association (MLA)
Surinlert, Piyaporn…[et al.]. Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts. Journal of Toxicology No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1190878
American Medical Association (AMA)
Surinlert, Piyaporn& Kongthong, Nitchamon& Watthanard, Mariam& Sae-lao, Thannicha& Sookbangnop, Piyawat& Pholpramool, Chumpol…[et al.]. Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts. Journal of Toxicology. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1190878
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1190878