Intermittent High Glucose Exacerbates A-FABP Activation and Inflammatory Response through TLR4-JNK Signaling in THP-1 Cells
المؤلفون المشاركون
Zhou, Zhi-Guang
Huang, Gan
Tang, Lin
Xiao, Yang
Li, Hui
Luo, Han-Ying
Liu, Qing
Zhong, Feng
Xu, Jun-Mei
Dai, Ru-Ping
Xu, Aimin
المصدر
Journal of Immunology Research
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-04-11
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background.
Glucose fluctuation confers additional risks on diabetes-related vascular diseases, but the underlying mechanisms are unknown.
Macrophage activation mediated by TLR4-JNK signaling plays an important role during the progress of diabetes.
In the present study, we hypothesize that glucose fluctuation results in macrophage inflammation through TLR4-JNK signaling pathways.
Methods.
THP-1 cells were treated with normal glucose (5 mM), constant high glucose (25 mM), and intermittent high glucose (rotation per 6 h in 5 mM or 25 mM) for 24 h.
The mRNA and protein expression levels of TLR4, p-JNK, and adipocyte fatty acid-binding protein (A-FABP) were determined, and the proinflammatory cytokines TNF-α and IL-1β were quantified.
Results.
In constant high glucose, TLR4 expression and JNK phosphorylation levels increased, and this effect was more pronounced in intermittent high glucose.
Accordingly, the expression of A-FABP and the release of the proinflammatory cytokines TNF-α and IL-1β also increased in response to constant high glucose, an effect that also was more evident in intermittent high glucose.
The inhibition of p-JNK by SP600125 did not attenuate TLR4 expression, but totally inhibited both A-FABP expression and the production of the proinflammatory cytokines TNF-α and IL-1β in both constant and intermittent high glucose.
Conclusions.
Intermittent high glucose potentiates A-FABP activation and inflammatory responses via TLR4/p-JNK signaling in THP-1 cells.
These findings suggest a more detrimental impact of glucose fluctuation on macrophage inflammation in diabetes-related vascular diseases than thus far generally assumed.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Hui& Luo, Han-Ying& Liu, Qing& Xiao, Yang& Tang, Lin& Zhong, Feng…[et al.]. 2018. Intermittent High Glucose Exacerbates A-FABP Activation and Inflammatory Response through TLR4-JNK Signaling in THP-1 Cells. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1191577
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Hui…[et al.]. Intermittent High Glucose Exacerbates A-FABP Activation and Inflammatory Response through TLR4-JNK Signaling in THP-1 Cells. Journal of Immunology Research No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1191577
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Hui& Luo, Han-Ying& Liu, Qing& Xiao, Yang& Tang, Lin& Zhong, Feng…[et al.]. Intermittent High Glucose Exacerbates A-FABP Activation and Inflammatory Response through TLR4-JNK Signaling in THP-1 Cells. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1191577
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1191577
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر