Intermittent High Glucose Exacerbates A-FABP Activation and Inflammatory Response through TLR4-JNK Signaling in THP-1 Cells
Joint Authors
Zhou, Zhi-Guang
Huang, Gan
Tang, Lin
Xiao, Yang
Li, Hui
Luo, Han-Ying
Liu, Qing
Zhong, Feng
Xu, Jun-Mei
Dai, Ru-Ping
Xu, Aimin
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Glucose fluctuation confers additional risks on diabetes-related vascular diseases, but the underlying mechanisms are unknown.
Macrophage activation mediated by TLR4-JNK signaling plays an important role during the progress of diabetes.
In the present study, we hypothesize that glucose fluctuation results in macrophage inflammation through TLR4-JNK signaling pathways.
Methods.
THP-1 cells were treated with normal glucose (5 mM), constant high glucose (25 mM), and intermittent high glucose (rotation per 6 h in 5 mM or 25 mM) for 24 h.
The mRNA and protein expression levels of TLR4, p-JNK, and adipocyte fatty acid-binding protein (A-FABP) were determined, and the proinflammatory cytokines TNF-α and IL-1β were quantified.
Results.
In constant high glucose, TLR4 expression and JNK phosphorylation levels increased, and this effect was more pronounced in intermittent high glucose.
Accordingly, the expression of A-FABP and the release of the proinflammatory cytokines TNF-α and IL-1β also increased in response to constant high glucose, an effect that also was more evident in intermittent high glucose.
The inhibition of p-JNK by SP600125 did not attenuate TLR4 expression, but totally inhibited both A-FABP expression and the production of the proinflammatory cytokines TNF-α and IL-1β in both constant and intermittent high glucose.
Conclusions.
Intermittent high glucose potentiates A-FABP activation and inflammatory responses via TLR4/p-JNK signaling in THP-1 cells.
These findings suggest a more detrimental impact of glucose fluctuation on macrophage inflammation in diabetes-related vascular diseases than thus far generally assumed.
American Psychological Association (APA)
Li, Hui& Luo, Han-Ying& Liu, Qing& Xiao, Yang& Tang, Lin& Zhong, Feng…[et al.]. 2018. Intermittent High Glucose Exacerbates A-FABP Activation and Inflammatory Response through TLR4-JNK Signaling in THP-1 Cells. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1191577
Modern Language Association (MLA)
Li, Hui…[et al.]. Intermittent High Glucose Exacerbates A-FABP Activation and Inflammatory Response through TLR4-JNK Signaling in THP-1 Cells. Journal of Immunology Research No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1191577
American Medical Association (AMA)
Li, Hui& Luo, Han-Ying& Liu, Qing& Xiao, Yang& Tang, Lin& Zhong, Feng…[et al.]. Intermittent High Glucose Exacerbates A-FABP Activation and Inflammatory Response through TLR4-JNK Signaling in THP-1 Cells. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1191577
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1191577