Aberrant Expression of miR-362 Promotes Lung Cancer Metastasis through Downregulation of Sema3A
المؤلفون المشاركون
Luo, Dan
Zhang, Zheng
Zhang, Zhao
Li, Jia-Yue
Cui, Jian
Shi, Wen-Pu
Dong, Xi-Wen
Yuan, Lin
Lin, Peng
Chen, Zhi-Nan
Bian, Hui-Jie
Wang, Zi-Ling
المصدر
Journal of Immunology Research
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-08-01
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
miR-362 is a recently discovered member of the microRNA family, and it modulates a variety of physical activities and plays an important role in the occurrence and development of many tumors.
However, the biological functions of hsa-miR-362-5p in non-small-cell lung carcinoma (NSCLC) are unknown.
Transwell assay and colony formation were used to determine the migration, invasion, and proliferation of NSCLC cells in vitro.
A subcutaneous tumor model in nude mice was established to detect NSCLC tumor growth in vivo.
The direct binding of miR-362 to the 3′UTR of Semaphorin 3A (Sema3A) was confirmed by luciferase reporter assay.
In this study, we found that the level of miR-362 was higher in NSCLC tissues than in adjacent normal tissues and that the level of miR-362 expression was also elevated in five NSCLC cell lines (A549, 95-D, H1299, H292, and H460) relative to a human normal lung epithelial cell line (BEAS2B).
Furthermore, miR-362 promoted NSCLC cell invasion, migration, and colony formation in vitro and tumor formation in vivo.
Next, we identified the miR-362 target gene Sema3A, which is significantly correlated with metastasis.
Sema3A expression was increased in normal tissues relative to NSCLC tissues.
This result is consistent with the fact that miR-362 expression is negatively correlated with Sema3A expression in clinical tissue samples and indicated that miR-362 can regulate Sema3A expression in NSCLC cells and consequently affect NSCLC invasion, migration, and colony formation.
Taken together, these findings on the newly identified miR-362/Sema3A axis elucidate the molecular mechanism of NSCLC invasion and migration and could lead to a potential therapeutic target in NSCLC treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Luo, Dan& Zhang, Zheng& Zhang, Zhao& Li, Jia-Yue& Cui, Jian& Shi, Wen-Pu…[et al.]. 2018. Aberrant Expression of miR-362 Promotes Lung Cancer Metastasis through Downregulation of Sema3A. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1191669
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Luo, Dan…[et al.]. Aberrant Expression of miR-362 Promotes Lung Cancer Metastasis through Downregulation of Sema3A. Journal of Immunology Research No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1191669
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Luo, Dan& Zhang, Zheng& Zhang, Zhao& Li, Jia-Yue& Cui, Jian& Shi, Wen-Pu…[et al.]. Aberrant Expression of miR-362 Promotes Lung Cancer Metastasis through Downregulation of Sema3A. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1191669
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1191669
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر