Aberrant Expression of miR-362 Promotes Lung Cancer Metastasis through Downregulation of Sema3A
Joint Authors
Luo, Dan
Zhang, Zheng
Zhang, Zhao
Li, Jia-Yue
Cui, Jian
Shi, Wen-Pu
Dong, Xi-Wen
Yuan, Lin
Lin, Peng
Chen, Zhi-Nan
Bian, Hui-Jie
Wang, Zi-Ling
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
miR-362 is a recently discovered member of the microRNA family, and it modulates a variety of physical activities and plays an important role in the occurrence and development of many tumors.
However, the biological functions of hsa-miR-362-5p in non-small-cell lung carcinoma (NSCLC) are unknown.
Transwell assay and colony formation were used to determine the migration, invasion, and proliferation of NSCLC cells in vitro.
A subcutaneous tumor model in nude mice was established to detect NSCLC tumor growth in vivo.
The direct binding of miR-362 to the 3′UTR of Semaphorin 3A (Sema3A) was confirmed by luciferase reporter assay.
In this study, we found that the level of miR-362 was higher in NSCLC tissues than in adjacent normal tissues and that the level of miR-362 expression was also elevated in five NSCLC cell lines (A549, 95-D, H1299, H292, and H460) relative to a human normal lung epithelial cell line (BEAS2B).
Furthermore, miR-362 promoted NSCLC cell invasion, migration, and colony formation in vitro and tumor formation in vivo.
Next, we identified the miR-362 target gene Sema3A, which is significantly correlated with metastasis.
Sema3A expression was increased in normal tissues relative to NSCLC tissues.
This result is consistent with the fact that miR-362 expression is negatively correlated with Sema3A expression in clinical tissue samples and indicated that miR-362 can regulate Sema3A expression in NSCLC cells and consequently affect NSCLC invasion, migration, and colony formation.
Taken together, these findings on the newly identified miR-362/Sema3A axis elucidate the molecular mechanism of NSCLC invasion and migration and could lead to a potential therapeutic target in NSCLC treatment.
American Psychological Association (APA)
Luo, Dan& Zhang, Zheng& Zhang, Zhao& Li, Jia-Yue& Cui, Jian& Shi, Wen-Pu…[et al.]. 2018. Aberrant Expression of miR-362 Promotes Lung Cancer Metastasis through Downregulation of Sema3A. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1191669
Modern Language Association (MLA)
Luo, Dan…[et al.]. Aberrant Expression of miR-362 Promotes Lung Cancer Metastasis through Downregulation of Sema3A. Journal of Immunology Research No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1191669
American Medical Association (AMA)
Luo, Dan& Zhang, Zheng& Zhang, Zhao& Li, Jia-Yue& Cui, Jian& Shi, Wen-Pu…[et al.]. Aberrant Expression of miR-362 Promotes Lung Cancer Metastasis through Downregulation of Sema3A. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1191669
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1191669