IL-33 Protects Mice against DSS-Induced Chronic Colitis by Increasing Both Regulatory B Cell and Regulatory T Cell Responses as Well as Decreasing Th17 Cell Response

الملخص EN

Background.

Previously, we have reported that IL-33 functioned as a protective modulator in dextran sulfate sodium- (DSS-) induced chronic colitis by suppressing Th17 cell response in colon lamina propria and IL-33 induced both regulatory B cells (Bregs) and regulatory T cells (Tregs) in mesenteric lymph nodes (MLNs) of mice with DSS-induced acute colitis.

Moreover, we speculated that IL-33 would promote the Treg or Breg responses leading to the attenuation of DSS-induced chronic colitis.

So, we investigated the role of IL-33 on Bregs and Tregs in the MLN of DSS-induced chronic colitis mice.

Methods.

IL-33 was administered by intraperitoneal injection to mice with DSS-induced chronic colitis.

Clinical symptoms, colon length, and histological changes were determined.

The production of cytokines was measured by ELISA.

The T and B cell subsets were measured by flow cytometry.

The expression of mRNA of transcription factors was measured by quantitative real-time PCR.

Results.

We show that IL-33 treatment increases both Breg and Treg responses in the MLN of mice with DSS-induced chronic colitis.

Moreover, IL-33 treatment also decreases Th17 cell response in the MLN of mice with DSS-induced chronic colitis.

Conclusion.

Our data provide clear evidence that IL-33 plays a protective role in DSS-induced chronic colitis, which is closely related to increasing Breg and Treg responses in the MLN of mice as well as suppressing Th17 cell responses.