Response of Human Mesenchymal Stromal Cells from Periodontal Tissue to LPS Depends on the Purity but Not on the LPS Source

المؤلفون المشاركون

Schäffer, Christina
Moritz, Andreas
Andrukhov, Oleh
Behm, Christian
Blufstein, Alice
Abhari, Setareh Younes
Koch, Christoph
Gahn, Johannes
Rausch-Fan, Xiaohui

المصدر

Mediators of Inflammation

العدد

المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-17، 17ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2020-07-02

دولة النشر

مصر

عدد الصفحات

17

التخصصات الرئيسية

الأمراض

الملخص EN

Human periodontal ligament stromal cells (hPDLSCs) and gingival mesenchymal stromal cells (hGMSCs) are resident mesenchymal stromal cells (MSCs) of the periodontal tissue.

The lipopolysaccharide (LPS) from Porphyromonas gingivalis is structurally distinct from that of other Gram-negative bacteria, and earlier studies linked this structural difference to a distinct virulence activity and the ability to activate toll-like receptor 2 (TLR-2), besides TLR-4 as commonly occurring upon LPS challenge.

Later studies, in contrast, argue that TLR-2 activation by P.

gingivalis LPS is due to lipoprotein contamination.

In the present study, we aimed to define the influence of structure versus purity of P.

gingivalis LPS on the immune response of hPDLSCs and hGMSCs.

Cells were stimulated with commercially available “standard” P.

gingivalis LPS, “ultrapure” P.

gingivalis LPS, or “ultrapure” Escherichia coli LPS, and the expression of interleukin- (IL-) 8, IL-6, monocyte chemoattractant protein- (MCP-) 1, TLR-2, and TLR-4 was evaluated.

The contribution of TLR-4 to the LPS-induced response was assessed using the specific TLR-4 inhibitor TAK-242.

“Standard” P.

gingivalis LPS induced significantly higher IL-8, IL-6, and MCP-1 production compared to the “ultrapure” LPS preparations, with no significant difference detectable for “ultrapure” LPS from P.

gingivalis and E.

coli.

By using TAK-242, the response of hPDLSCs and hGMSCs to “ultrapure” LPS preparations was effectively inhibited to the levels comparable to those of nonstimulated controls.

In contrast, high levels of response to “standard” LPS were observed, even in the presence of TAK-242.

Our data show that the response of MSCs from periodontal tissue to LPS depends more on the purity of the LPS preparation than on the LPS source.

Even a small amount of contaminating lipoproteins can drastically enhance the hPDLSCs’ and hGMSCs; responsiveness to P.

gingivalis LPS, which might also contribute to the progression of periodontal disease.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Behm, Christian& Blufstein, Alice& Abhari, Setareh Younes& Koch, Christoph& Gahn, Johannes& Schäffer, Christina…[et al.]. 2020. Response of Human Mesenchymal Stromal Cells from Periodontal Tissue to LPS Depends on the Purity but Not on the LPS Source. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1192125

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Behm, Christian…[et al.]. Response of Human Mesenchymal Stromal Cells from Periodontal Tissue to LPS Depends on the Purity but Not on the LPS Source. Mediators of Inflammation No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1192125

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Behm, Christian& Blufstein, Alice& Abhari, Setareh Younes& Koch, Christoph& Gahn, Johannes& Schäffer, Christina…[et al.]. Response of Human Mesenchymal Stromal Cells from Periodontal Tissue to LPS Depends on the Purity but Not on the LPS Source. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1192125

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1192125