Response of Human Mesenchymal Stromal Cells from Periodontal Tissue to LPS Depends on the Purity but Not on the LPS Source
Joint Authors
Schäffer, Christina
Moritz, Andreas
Andrukhov, Oleh
Behm, Christian
Blufstein, Alice
Abhari, Setareh Younes
Koch, Christoph
Gahn, Johannes
Rausch-Fan, Xiaohui
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-02
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Human periodontal ligament stromal cells (hPDLSCs) and gingival mesenchymal stromal cells (hGMSCs) are resident mesenchymal stromal cells (MSCs) of the periodontal tissue.
The lipopolysaccharide (LPS) from Porphyromonas gingivalis is structurally distinct from that of other Gram-negative bacteria, and earlier studies linked this structural difference to a distinct virulence activity and the ability to activate toll-like receptor 2 (TLR-2), besides TLR-4 as commonly occurring upon LPS challenge.
Later studies, in contrast, argue that TLR-2 activation by P.
gingivalis LPS is due to lipoprotein contamination.
In the present study, we aimed to define the influence of structure versus purity of P.
gingivalis LPS on the immune response of hPDLSCs and hGMSCs.
Cells were stimulated with commercially available “standard” P.
gingivalis LPS, “ultrapure” P.
gingivalis LPS, or “ultrapure” Escherichia coli LPS, and the expression of interleukin- (IL-) 8, IL-6, monocyte chemoattractant protein- (MCP-) 1, TLR-2, and TLR-4 was evaluated.
The contribution of TLR-4 to the LPS-induced response was assessed using the specific TLR-4 inhibitor TAK-242.
“Standard” P.
gingivalis LPS induced significantly higher IL-8, IL-6, and MCP-1 production compared to the “ultrapure” LPS preparations, with no significant difference detectable for “ultrapure” LPS from P.
gingivalis and E.
coli.
By using TAK-242, the response of hPDLSCs and hGMSCs to “ultrapure” LPS preparations was effectively inhibited to the levels comparable to those of nonstimulated controls.
In contrast, high levels of response to “standard” LPS were observed, even in the presence of TAK-242.
Our data show that the response of MSCs from periodontal tissue to LPS depends more on the purity of the LPS preparation than on the LPS source.
Even a small amount of contaminating lipoproteins can drastically enhance the hPDLSCs’ and hGMSCs; responsiveness to P.
gingivalis LPS, which might also contribute to the progression of periodontal disease.
American Psychological Association (APA)
Behm, Christian& Blufstein, Alice& Abhari, Setareh Younes& Koch, Christoph& Gahn, Johannes& Schäffer, Christina…[et al.]. 2020. Response of Human Mesenchymal Stromal Cells from Periodontal Tissue to LPS Depends on the Purity but Not on the LPS Source. Mediators of Inflammation،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1192125
Modern Language Association (MLA)
Behm, Christian…[et al.]. Response of Human Mesenchymal Stromal Cells from Periodontal Tissue to LPS Depends on the Purity but Not on the LPS Source. Mediators of Inflammation No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1192125
American Medical Association (AMA)
Behm, Christian& Blufstein, Alice& Abhari, Setareh Younes& Koch, Christoph& Gahn, Johannes& Schäffer, Christina…[et al.]. Response of Human Mesenchymal Stromal Cells from Periodontal Tissue to LPS Depends on the Purity but Not on the LPS Source. Mediators of Inflammation. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1192125
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192125