Luteolin Induces Apoptosis and Autophagy in Mouse Macrophage ANA-1 Cells via the Bcl-2 Pathway
المؤلفون المشاركون
Zhu, Guoqiang
Liao, Yuexia
Xu, Yang
Cao, Mengyao
Huan, Yuanyuan
Zhu, Lei
Jiang, Ye
Shen, Weigan
المصدر
Journal of Immunology Research
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-08-30
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Plants rich in luteolin have been used as Chinese traditional medicines for inflammatory diseases, hypertension, and cancer.
However, little is known about the effect of luteolin on the apoptosis or autophagy of the macrophages.
In this study, mouse macrophage ANA-1 cells were incubated with different concentrations of luteolin.
The viability of the cells was determined by an MTT assay, apoptosis was determined by flow cytometric analysis, the level of cell autophagy was observed by confocal microscopy, and the expression levels of apoptotic or autophagic and antiapoptotic or antiautophagic proteins were detected by Western blot analysis.
The results showed that luteolin decreased the viability of ANA-1 cells and induced apoptosis and autophagy.
Luteolin induced apoptosis accompanied by downregulation of the expression of Bcl-2 and upregulation of the expression of caspase 3 and caspase 8.
And luteolin increased FITC-LC3 punctate fluorescence accompanied by the increased expression levels of LC3-I, ATG7, and ATG12, while it suppressed the expression level of Beclin-1.
Luteolin treatment resulted in obvious activation of the p38, JNK, and Akt signaling pathways, which is important in modulating apoptosis and autophagy.
Thus, we concluded that luteolin induced the apoptosis and autophagy of ANA-1 cells most likely by regulating the p38, JNK, and Akt pathways, inhibiting the activity of Bcl-2 and Beclin-1 and upregulating caspase 3 and caspase 8 expression.
These results provide novel insights into a therapeutic strategy to prevent and possibly treat macrophage-related diseases through luteolin-induced apoptosis and autophagy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liao, Yuexia& Xu, Yang& Cao, Mengyao& Huan, Yuanyuan& Zhu, Lei& Jiang, Ye…[et al.]. 2018. Luteolin Induces Apoptosis and Autophagy in Mouse Macrophage ANA-1 Cells via the Bcl-2 Pathway. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1192370
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liao, Yuexia…[et al.]. Luteolin Induces Apoptosis and Autophagy in Mouse Macrophage ANA-1 Cells via the Bcl-2 Pathway. Journal of Immunology Research No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1192370
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liao, Yuexia& Xu, Yang& Cao, Mengyao& Huan, Yuanyuan& Zhu, Lei& Jiang, Ye…[et al.]. Luteolin Induces Apoptosis and Autophagy in Mouse Macrophage ANA-1 Cells via the Bcl-2 Pathway. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1192370
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1192370
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر