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Luteolin Induces Apoptosis and Autophagy in Mouse Macrophage ANA-1 Cells via the Bcl-2 Pathway
Joint Authors
Zhu, Guoqiang
Liao, Yuexia
Xu, Yang
Cao, Mengyao
Huan, Yuanyuan
Zhu, Lei
Jiang, Ye
Shen, Weigan
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Plants rich in luteolin have been used as Chinese traditional medicines for inflammatory diseases, hypertension, and cancer.
However, little is known about the effect of luteolin on the apoptosis or autophagy of the macrophages.
In this study, mouse macrophage ANA-1 cells were incubated with different concentrations of luteolin.
The viability of the cells was determined by an MTT assay, apoptosis was determined by flow cytometric analysis, the level of cell autophagy was observed by confocal microscopy, and the expression levels of apoptotic or autophagic and antiapoptotic or antiautophagic proteins were detected by Western blot analysis.
The results showed that luteolin decreased the viability of ANA-1 cells and induced apoptosis and autophagy.
Luteolin induced apoptosis accompanied by downregulation of the expression of Bcl-2 and upregulation of the expression of caspase 3 and caspase 8.
And luteolin increased FITC-LC3 punctate fluorescence accompanied by the increased expression levels of LC3-I, ATG7, and ATG12, while it suppressed the expression level of Beclin-1.
Luteolin treatment resulted in obvious activation of the p38, JNK, and Akt signaling pathways, which is important in modulating apoptosis and autophagy.
Thus, we concluded that luteolin induced the apoptosis and autophagy of ANA-1 cells most likely by regulating the p38, JNK, and Akt pathways, inhibiting the activity of Bcl-2 and Beclin-1 and upregulating caspase 3 and caspase 8 expression.
These results provide novel insights into a therapeutic strategy to prevent and possibly treat macrophage-related diseases through luteolin-induced apoptosis and autophagy.
American Psychological Association (APA)
Liao, Yuexia& Xu, Yang& Cao, Mengyao& Huan, Yuanyuan& Zhu, Lei& Jiang, Ye…[et al.]. 2018. Luteolin Induces Apoptosis and Autophagy in Mouse Macrophage ANA-1 Cells via the Bcl-2 Pathway. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1192370
Modern Language Association (MLA)
Liao, Yuexia…[et al.]. Luteolin Induces Apoptosis and Autophagy in Mouse Macrophage ANA-1 Cells via the Bcl-2 Pathway. Journal of Immunology Research No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1192370
American Medical Association (AMA)
Liao, Yuexia& Xu, Yang& Cao, Mengyao& Huan, Yuanyuan& Zhu, Lei& Jiang, Ye…[et al.]. Luteolin Induces Apoptosis and Autophagy in Mouse Macrophage ANA-1 Cells via the Bcl-2 Pathway. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1192370
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192370