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A Quantitative Method for Detecting Ara h 2 by Generation and Utilization of Monoclonal Antibodies
المؤلفون المشاركون
Zou, Zehong
Chen, Huifang
Tao, Ailin
المصدر
Journal of Immunology Research
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-05-06
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Peanut (Arachis hypogaea) is one of the most common food allergens that can induce fatal anaphylaxis, and Ara h 2 is one of the major allergen components involved in peanut allergy.
The aim of this study was to develop a quantitative method for detecting peanut allergen using monoclonal antibodies against Ara h 2.
The splenocytes of immunized mice were fused with myeloma cells (SP2/0), and stable mAb-producing clones were obtained by limiting dilution.
mAbs against Ara h 2 were isolated from mouse ascites, and specificity was confirmed by immunoblotting.
Five mAbs with high purity and specific reactivity were obtained, which were referred to as 1-2E10, 2-1D5, 3-1C5, 4-1C2, and 5-1G4, respectively.
After screening different mAb combinations for development of a sandwich ELISA, we selected 5-1G4 as the capture antibody and 1-2E10 as the detection antibody for the measurement of Ara h 2 from which an optimal correlation between the Ara h 2 concentration and the OD value was obtained.
This sandwich ELISA could specifically detect Ara h 2 in peanut extract at concentrations as low as 5 ng/mL and up to 10 μg/mL.
These mAbs can, therefore, serve as quantitative diagnostic reagents for peanut and peanut product risk assessment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Huifang& Zou, Zehong& Tao, Ailin. 2018. A Quantitative Method for Detecting Ara h 2 by Generation and Utilization of Monoclonal Antibodies. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1192420
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Huifang…[et al.]. A Quantitative Method for Detecting Ara h 2 by Generation and Utilization of Monoclonal Antibodies. Journal of Immunology Research No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1192420
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Huifang& Zou, Zehong& Tao, Ailin. A Quantitative Method for Detecting Ara h 2 by Generation and Utilization of Monoclonal Antibodies. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1192420
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1192420
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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