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A Quantitative Method for Detecting Ara h 2 by Generation and Utilization of Monoclonal Antibodies
Joint Authors
Zou, Zehong
Chen, Huifang
Tao, Ailin
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Peanut (Arachis hypogaea) is one of the most common food allergens that can induce fatal anaphylaxis, and Ara h 2 is one of the major allergen components involved in peanut allergy.
The aim of this study was to develop a quantitative method for detecting peanut allergen using monoclonal antibodies against Ara h 2.
The splenocytes of immunized mice were fused with myeloma cells (SP2/0), and stable mAb-producing clones were obtained by limiting dilution.
mAbs against Ara h 2 were isolated from mouse ascites, and specificity was confirmed by immunoblotting.
Five mAbs with high purity and specific reactivity were obtained, which were referred to as 1-2E10, 2-1D5, 3-1C5, 4-1C2, and 5-1G4, respectively.
After screening different mAb combinations for development of a sandwich ELISA, we selected 5-1G4 as the capture antibody and 1-2E10 as the detection antibody for the measurement of Ara h 2 from which an optimal correlation between the Ara h 2 concentration and the OD value was obtained.
This sandwich ELISA could specifically detect Ara h 2 in peanut extract at concentrations as low as 5 ng/mL and up to 10 μg/mL.
These mAbs can, therefore, serve as quantitative diagnostic reagents for peanut and peanut product risk assessment.
American Psychological Association (APA)
Chen, Huifang& Zou, Zehong& Tao, Ailin. 2018. A Quantitative Method for Detecting Ara h 2 by Generation and Utilization of Monoclonal Antibodies. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1192420
Modern Language Association (MLA)
Chen, Huifang…[et al.]. A Quantitative Method for Detecting Ara h 2 by Generation and Utilization of Monoclonal Antibodies. Journal of Immunology Research No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1192420
American Medical Association (AMA)
Chen, Huifang& Zou, Zehong& Tao, Ailin. A Quantitative Method for Detecting Ara h 2 by Generation and Utilization of Monoclonal Antibodies. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1192420
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192420