Involvement of Oxidative Stress and the Epidermal Growth Factor Receptor in Diesel Exhaust Particle-Induced Expression of Inflammatory Mediators in Human Mononuclear Cells
المؤلفون المشاركون
Wu, Weidong
Li, Fangfang
An, Zhen
Li, Haibin
Gao, Xia
Wang, Gui
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-11-15
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Exposure to diesel exhaust particles (DEPs) has been associated with increased incidence of cardiopulmonary diseases.
This study is aimed at examining the proinflammatory effects of DEP on primary human peripheral blood mononuclear cells (PBMC) and the underlying mechanisms using a human mononuclear cell line, THP-1.
DEPs were incubated with the PBMC and THP-1 cells for 24 h, respectively.
The supernatants were collected and subjected to measurement of proinflammatory mediators including interleukin 8 (IL-8) or tumor necrosis factor α (TNFα) by ELISA.
Levels of reactive oxygen species (ROS) were determined using flow cytometry.
Phosphorylation of the epidermal growth factor receptor (EGFR) was examined with immunoblotting.
Exposure to DEP induced a concentration-dependent increase in the expression of IL-8 and TNFα in the PBMC and THP-1 cells.
Further mechanistic studies with THP-1 cells indicated that DEP stimulation increased intracellular levels of ROS, an indicator of oxidative stress, and phosphorylation of the EGFR, indicative of EGFR activation.
Pretreatment of THP-1 cells with the antioxidant N-acetyl-L-cysteine (NAC) markedly blunted DEP-induced EGFR phosphorylation, indicating that oxidative stress was involved in DEP-induced EGFR activation.
Furthermore, the pretreatment of THP-1 cells with either NAC or a selective EGFR inhibitor significantly blocked DEP-induced IL-8 expression, implying that oxidative stress and subsequent EGFR activation mediated DEP-induced inflammatory response.
In summary, DEP stimulation increases the expression of proinflammatory mediators in human mononuclear cells, which is regulated by oxidative stress-EGFR signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Fangfang& An, Zhen& Li, Haibin& Gao, Xia& Wang, Gui& Wu, Weidong. 2019. Involvement of Oxidative Stress and the Epidermal Growth Factor Receptor in Diesel Exhaust Particle-Induced Expression of Inflammatory Mediators in Human Mononuclear Cells. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1192809
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Fangfang…[et al.]. Involvement of Oxidative Stress and the Epidermal Growth Factor Receptor in Diesel Exhaust Particle-Induced Expression of Inflammatory Mediators in Human Mononuclear Cells. Mediators of Inflammation No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1192809
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Fangfang& An, Zhen& Li, Haibin& Gao, Xia& Wang, Gui& Wu, Weidong. Involvement of Oxidative Stress and the Epidermal Growth Factor Receptor in Diesel Exhaust Particle-Induced Expression of Inflammatory Mediators in Human Mononuclear Cells. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1192809
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1192809
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر