Involvement of Oxidative Stress and the Epidermal Growth Factor Receptor in Diesel Exhaust Particle-Induced Expression of Inflammatory Mediators in Human Mononuclear Cells
Joint Authors
Wu, Weidong
Li, Fangfang
An, Zhen
Li, Haibin
Gao, Xia
Wang, Gui
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Exposure to diesel exhaust particles (DEPs) has been associated with increased incidence of cardiopulmonary diseases.
This study is aimed at examining the proinflammatory effects of DEP on primary human peripheral blood mononuclear cells (PBMC) and the underlying mechanisms using a human mononuclear cell line, THP-1.
DEPs were incubated with the PBMC and THP-1 cells for 24 h, respectively.
The supernatants were collected and subjected to measurement of proinflammatory mediators including interleukin 8 (IL-8) or tumor necrosis factor α (TNFα) by ELISA.
Levels of reactive oxygen species (ROS) were determined using flow cytometry.
Phosphorylation of the epidermal growth factor receptor (EGFR) was examined with immunoblotting.
Exposure to DEP induced a concentration-dependent increase in the expression of IL-8 and TNFα in the PBMC and THP-1 cells.
Further mechanistic studies with THP-1 cells indicated that DEP stimulation increased intracellular levels of ROS, an indicator of oxidative stress, and phosphorylation of the EGFR, indicative of EGFR activation.
Pretreatment of THP-1 cells with the antioxidant N-acetyl-L-cysteine (NAC) markedly blunted DEP-induced EGFR phosphorylation, indicating that oxidative stress was involved in DEP-induced EGFR activation.
Furthermore, the pretreatment of THP-1 cells with either NAC or a selective EGFR inhibitor significantly blocked DEP-induced IL-8 expression, implying that oxidative stress and subsequent EGFR activation mediated DEP-induced inflammatory response.
In summary, DEP stimulation increases the expression of proinflammatory mediators in human mononuclear cells, which is regulated by oxidative stress-EGFR signaling pathway.
American Psychological Association (APA)
Li, Fangfang& An, Zhen& Li, Haibin& Gao, Xia& Wang, Gui& Wu, Weidong. 2019. Involvement of Oxidative Stress and the Epidermal Growth Factor Receptor in Diesel Exhaust Particle-Induced Expression of Inflammatory Mediators in Human Mononuclear Cells. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1192809
Modern Language Association (MLA)
Li, Fangfang…[et al.]. Involvement of Oxidative Stress and the Epidermal Growth Factor Receptor in Diesel Exhaust Particle-Induced Expression of Inflammatory Mediators in Human Mononuclear Cells. Mediators of Inflammation No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1192809
American Medical Association (AMA)
Li, Fangfang& An, Zhen& Li, Haibin& Gao, Xia& Wang, Gui& Wu, Weidong. Involvement of Oxidative Stress and the Epidermal Growth Factor Receptor in Diesel Exhaust Particle-Induced Expression of Inflammatory Mediators in Human Mononuclear Cells. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1192809
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1192809