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Proteomic Analysis of HDAC3 Selective Inhibitor in the Regulation of Inflammatory Response of Primary Microglia
المؤلفون المشاركون
Xu, Yun
Xia, Mingxu
Zhao, Qiuchen
Zhang, He
Chen, Yanting
Yuan, Zengqiang
Zhang, Meijuan
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-02-15
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
HDAC3 has been shown to regulate inflammation.
However, the role of HDAC3 in primary microglia is largely unknown.
RGFP966 is a newly discovered selective HDAC3 inhibitor.
In this study, we used protein mass spectrometry to analyze protein alterations in LPS-treated primary microglia with the application of RGFP966.
Generally, about 2000 proteins were studied.
168 of 444 (37.8%) LPS-induced proteins were significantly reduced with the treatment of RGFP966, which mainly concentrated on Toll-like receptor signaling pathway.
In this regard, we selected Toll-like receptor 2 (TLR2), TLR3, TLR6, MAPK p38, CD36, and spleen tyrosine kinase (SYK) for further validation and found that they were all significantly upregulated after LPS stimulation and downregulated in the presence of RGFP966.
Additionally, RGFP966 inhibited supernatant tumor necrosis factor (TNF)-α and Interleukin 6 (IL-6) concentrations.
Activation of STAT3 and STAT5 was partially blocked by RGFP966 at 2 h after LPS-stimulation.
The fluorescence intensity of CD16/32 was significantly decreased in LPS + RGFP966-treated group.
In conclusion, our data provided a hint that RGFP966 may be a potential therapeutic medication combating microglia activation and inflammatory response in central nervous system, which was probably related to its repressive impacts on TLR signaling pathways and STAT3/STAT5 pathways.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xia, Mingxu& Zhao, Qiuchen& Zhang, He& Chen, Yanting& Yuan, Zengqiang& Xu, Yun…[et al.]. 2017. Proteomic Analysis of HDAC3 Selective Inhibitor in the Regulation of Inflammatory Response of Primary Microglia. Neural Plasticity،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1193124
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xia, Mingxu…[et al.]. Proteomic Analysis of HDAC3 Selective Inhibitor in the Regulation of Inflammatory Response of Primary Microglia. Neural Plasticity No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1193124
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xia, Mingxu& Zhao, Qiuchen& Zhang, He& Chen, Yanting& Yuan, Zengqiang& Xu, Yun…[et al.]. Proteomic Analysis of HDAC3 Selective Inhibitor in the Regulation of Inflammatory Response of Primary Microglia. Neural Plasticity. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1193124
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1193124
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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