![](/images/graphics-bg.png)
Proteomic Analysis of HDAC3 Selective Inhibitor in the Regulation of Inflammatory Response of Primary Microglia
Joint Authors
Xu, Yun
Xia, Mingxu
Zhao, Qiuchen
Zhang, He
Chen, Yanting
Yuan, Zengqiang
Zhang, Meijuan
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-15
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
HDAC3 has been shown to regulate inflammation.
However, the role of HDAC3 in primary microglia is largely unknown.
RGFP966 is a newly discovered selective HDAC3 inhibitor.
In this study, we used protein mass spectrometry to analyze protein alterations in LPS-treated primary microglia with the application of RGFP966.
Generally, about 2000 proteins were studied.
168 of 444 (37.8%) LPS-induced proteins were significantly reduced with the treatment of RGFP966, which mainly concentrated on Toll-like receptor signaling pathway.
In this regard, we selected Toll-like receptor 2 (TLR2), TLR3, TLR6, MAPK p38, CD36, and spleen tyrosine kinase (SYK) for further validation and found that they were all significantly upregulated after LPS stimulation and downregulated in the presence of RGFP966.
Additionally, RGFP966 inhibited supernatant tumor necrosis factor (TNF)-α and Interleukin 6 (IL-6) concentrations.
Activation of STAT3 and STAT5 was partially blocked by RGFP966 at 2 h after LPS-stimulation.
The fluorescence intensity of CD16/32 was significantly decreased in LPS + RGFP966-treated group.
In conclusion, our data provided a hint that RGFP966 may be a potential therapeutic medication combating microglia activation and inflammatory response in central nervous system, which was probably related to its repressive impacts on TLR signaling pathways and STAT3/STAT5 pathways.
American Psychological Association (APA)
Xia, Mingxu& Zhao, Qiuchen& Zhang, He& Chen, Yanting& Yuan, Zengqiang& Xu, Yun…[et al.]. 2017. Proteomic Analysis of HDAC3 Selective Inhibitor in the Regulation of Inflammatory Response of Primary Microglia. Neural Plasticity،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1193124
Modern Language Association (MLA)
Xia, Mingxu…[et al.]. Proteomic Analysis of HDAC3 Selective Inhibitor in the Regulation of Inflammatory Response of Primary Microglia. Neural Plasticity No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1193124
American Medical Association (AMA)
Xia, Mingxu& Zhao, Qiuchen& Zhang, He& Chen, Yanting& Yuan, Zengqiang& Xu, Yun…[et al.]. Proteomic Analysis of HDAC3 Selective Inhibitor in the Regulation of Inflammatory Response of Primary Microglia. Neural Plasticity. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1193124
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193124