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Analysis of Immune Responses in Mice Orally Immunized with Recombinant pMG36e-SP-TSOL18Lactococcus lactis and pMG36e-TSOL18Lactococcus lactis Vaccines of Taenia solium
المؤلفون المشاركون
Zhou, Bi Ying
Sun, Jun Chao
Li, Xiang
Zhang, Yue
Luo, Bo
Jiang, Nan
Liu, Mei Chen
المصدر
Journal of Immunology Research
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-11-18
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Cysticercosis is a cosmopolitan zoonotic parasitic disease infected by larval of Taenia solium (T.
solium).
Several drugs for the treatment of cysticercosis, such as praziquantel, albendazole, and mebendazole, have certain toxicity and side effects.
Considering that there is no vaccine available, we studied a new vaccine for cysticercosis in this study.
The complete TSOL18 gene and the optimized SP-TSOL18 gene fragments were obtained using PCR-based accurate synthesis method.
The secretory and intracellular recombinant pMG36e-SP-TSOL18/Lactococcus lactis (L.
lactis) and pMG36e-TSOL18/L.
lactis vaccines of T.
solium were prepared.
Immune responses in mice orally immunized with these two recombinant L.
lactis vaccines were analyzed by the determination of specific antibodies (IgG, IgG1, IgG2a, and sIgA) in serum, spleen lymphocyte proliferation, and cytokines (IL-2, IFN-γ, IL-4, and IL-10) in spleen lymphocyte culture supernatant.
Our results showed that, after the first immunization, in these two recombinant L.
lactis vaccine groups, the levels of serum specific IgG, IgG2a, and IgG1 increased on 14–56 d and reached the highest level on days 42, 42, and 28, respectively.
The level of specific sIgA of intestinal mucosa also increased on 14–56 d and reached the highest level on day 42.
The level of spleen lymphocyte proliferation increased on 14–56 d and reached the highest level on day 42.
The levels of IL-2, IFN-γ, IL-4, and IL-10 in spleen lymphocyte culture supernatant increased on 14–56 d and reached the highest level on days 42, 42, 28, and 28, respectively.
These results indicated that the recombinant pMG36e-SP-TSOL18/L.
lactis and pMG36e-TSOL18/L.
lactis vaccines can induce specific cellular, humoral, and mucosal immune responses in mice with oral vaccination.
More importantly, the recombinant pMG36e-SP-TSOL18/L.
lactis vaccine has a better immune effect.
In summary, these results demonstrated the possibility of using L.
lactis strain as a vector to deliver protective antigens of T.
solium.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhou, Bi Ying& Sun, Jun Chao& Li, Xiang& Zhang, Yue& Luo, Bo& Jiang, Nan…[et al.]. 2018. Analysis of Immune Responses in Mice Orally Immunized with Recombinant pMG36e-SP-TSOL18Lactococcus lactis and pMG36e-TSOL18Lactococcus lactis Vaccines of Taenia solium. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1193266
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhou, Bi Ying…[et al.]. Analysis of Immune Responses in Mice Orally Immunized with Recombinant pMG36e-SP-TSOL18Lactococcus lactis and pMG36e-TSOL18Lactococcus lactis Vaccines of Taenia solium. Journal of Immunology Research No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1193266
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhou, Bi Ying& Sun, Jun Chao& Li, Xiang& Zhang, Yue& Luo, Bo& Jiang, Nan…[et al.]. Analysis of Immune Responses in Mice Orally Immunized with Recombinant pMG36e-SP-TSOL18Lactococcus lactis and pMG36e-TSOL18Lactococcus lactis Vaccines of Taenia solium. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1193266
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1193266
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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