Analysis of Immune Responses in Mice Orally Immunized with Recombinant pMG36e-SP-TSOL18Lactococcus lactis and pMG36e-TSOL18Lactococcus lactis Vaccines of Taenia solium
Joint Authors
Zhou, Bi Ying
Sun, Jun Chao
Li, Xiang
Zhang, Yue
Luo, Bo
Jiang, Nan
Liu, Mei Chen
Source
Journal of Immunology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-18
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Cysticercosis is a cosmopolitan zoonotic parasitic disease infected by larval of Taenia solium (T.
solium).
Several drugs for the treatment of cysticercosis, such as praziquantel, albendazole, and mebendazole, have certain toxicity and side effects.
Considering that there is no vaccine available, we studied a new vaccine for cysticercosis in this study.
The complete TSOL18 gene and the optimized SP-TSOL18 gene fragments were obtained using PCR-based accurate synthesis method.
The secretory and intracellular recombinant pMG36e-SP-TSOL18/Lactococcus lactis (L.
lactis) and pMG36e-TSOL18/L.
lactis vaccines of T.
solium were prepared.
Immune responses in mice orally immunized with these two recombinant L.
lactis vaccines were analyzed by the determination of specific antibodies (IgG, IgG1, IgG2a, and sIgA) in serum, spleen lymphocyte proliferation, and cytokines (IL-2, IFN-γ, IL-4, and IL-10) in spleen lymphocyte culture supernatant.
Our results showed that, after the first immunization, in these two recombinant L.
lactis vaccine groups, the levels of serum specific IgG, IgG2a, and IgG1 increased on 14–56 d and reached the highest level on days 42, 42, and 28, respectively.
The level of specific sIgA of intestinal mucosa also increased on 14–56 d and reached the highest level on day 42.
The level of spleen lymphocyte proliferation increased on 14–56 d and reached the highest level on day 42.
The levels of IL-2, IFN-γ, IL-4, and IL-10 in spleen lymphocyte culture supernatant increased on 14–56 d and reached the highest level on days 42, 42, 28, and 28, respectively.
These results indicated that the recombinant pMG36e-SP-TSOL18/L.
lactis and pMG36e-TSOL18/L.
lactis vaccines can induce specific cellular, humoral, and mucosal immune responses in mice with oral vaccination.
More importantly, the recombinant pMG36e-SP-TSOL18/L.
lactis vaccine has a better immune effect.
In summary, these results demonstrated the possibility of using L.
lactis strain as a vector to deliver protective antigens of T.
solium.
American Psychological Association (APA)
Zhou, Bi Ying& Sun, Jun Chao& Li, Xiang& Zhang, Yue& Luo, Bo& Jiang, Nan…[et al.]. 2018. Analysis of Immune Responses in Mice Orally Immunized with Recombinant pMG36e-SP-TSOL18Lactococcus lactis and pMG36e-TSOL18Lactococcus lactis Vaccines of Taenia solium. Journal of Immunology Research،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1193266
Modern Language Association (MLA)
Zhou, Bi Ying…[et al.]. Analysis of Immune Responses in Mice Orally Immunized with Recombinant pMG36e-SP-TSOL18Lactococcus lactis and pMG36e-TSOL18Lactococcus lactis Vaccines of Taenia solium. Journal of Immunology Research No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1193266
American Medical Association (AMA)
Zhou, Bi Ying& Sun, Jun Chao& Li, Xiang& Zhang, Yue& Luo, Bo& Jiang, Nan…[et al.]. Analysis of Immune Responses in Mice Orally Immunized with Recombinant pMG36e-SP-TSOL18Lactococcus lactis and pMG36e-TSOL18Lactococcus lactis Vaccines of Taenia solium. Journal of Immunology Research. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1193266
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193266