Tocotrienol-Rich Fraction Ameliorates Antioxidant Defense Mechanisms and Improves Replicative Senescence-Associated Oxidative Stress in Human Myoblasts
المؤلفون المشاركون
Mohd Yusof, Yasmin Anum
Makpol, Suzana
Abdul Karim, Norwahidah
Ngah, Wan Zurinah Wan
Khor, Shy Cian
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-01-24
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
During aging, oxidative stress affects the normal function of satellite cells, with consequent regeneration defects that lead to sarcopenia.
This study aimed to evaluate tocotrienol-rich fraction (TRF) modulation in reestablishing the oxidative status of myoblasts during replicative senescence and to compare the effects of TRF with other antioxidants (α-tocopherol (ATF) and N-acetyl-cysteine (NAC)).
Primary human myoblasts were cultured to young, presenescent, and senescent phases.
The cells were treated with antioxidants for 24 h, followed by the assessment of free radical generation, lipid peroxidation, antioxidant enzyme mRNA expression and activities, and the ratio of reduced to oxidized glutathione.
Our data showed that replicative senescence increased reactive oxygen species (ROS) generation and lipid peroxidation in myoblasts.
Treatment with TRF significantly diminished ROS production and decreased lipid peroxidation in senescent myoblasts.
Moreover, the gene expression of superoxide dismutase (SOD2), catalase (CAT), and glutathione peroxidase (GPX1) was modulated by TRF treatment, with increased activity of superoxide dismutase and catalase and reduced glutathione peroxidase in senescent myoblasts.
In comparison to ATF and NAC, TRF was more efficient in heightening the antioxidant capacity and reducing free radical insults.
These results suggested that TRF is able to ameliorate antioxidant defense mechanisms and improves replicative senescence-associated oxidative stress in myoblasts.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Khor, Shy Cian& Ngah, Wan Zurinah Wan& Mohd Yusof, Yasmin Anum& Abdul Karim, Norwahidah& Makpol, Suzana. 2017. Tocotrienol-Rich Fraction Ameliorates Antioxidant Defense Mechanisms and Improves Replicative Senescence-Associated Oxidative Stress in Human Myoblasts. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-17.
https://search.emarefa.net/detail/BIM-1194465
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Khor, Shy Cian…[et al.]. Tocotrienol-Rich Fraction Ameliorates Antioxidant Defense Mechanisms and Improves Replicative Senescence-Associated Oxidative Stress in Human Myoblasts. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-17.
https://search.emarefa.net/detail/BIM-1194465
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Khor, Shy Cian& Ngah, Wan Zurinah Wan& Mohd Yusof, Yasmin Anum& Abdul Karim, Norwahidah& Makpol, Suzana. Tocotrienol-Rich Fraction Ameliorates Antioxidant Defense Mechanisms and Improves Replicative Senescence-Associated Oxidative Stress in Human Myoblasts. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-17.
https://search.emarefa.net/detail/BIM-1194465
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1194465
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر