Tocotrienol-Rich Fraction Ameliorates Antioxidant Defense Mechanisms and Improves Replicative Senescence-Associated Oxidative Stress in Human Myoblasts
Joint Authors
Mohd Yusof, Yasmin Anum
Makpol, Suzana
Abdul Karim, Norwahidah
Ngah, Wan Zurinah Wan
Khor, Shy Cian
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-24
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
During aging, oxidative stress affects the normal function of satellite cells, with consequent regeneration defects that lead to sarcopenia.
This study aimed to evaluate tocotrienol-rich fraction (TRF) modulation in reestablishing the oxidative status of myoblasts during replicative senescence and to compare the effects of TRF with other antioxidants (α-tocopherol (ATF) and N-acetyl-cysteine (NAC)).
Primary human myoblasts were cultured to young, presenescent, and senescent phases.
The cells were treated with antioxidants for 24 h, followed by the assessment of free radical generation, lipid peroxidation, antioxidant enzyme mRNA expression and activities, and the ratio of reduced to oxidized glutathione.
Our data showed that replicative senescence increased reactive oxygen species (ROS) generation and lipid peroxidation in myoblasts.
Treatment with TRF significantly diminished ROS production and decreased lipid peroxidation in senescent myoblasts.
Moreover, the gene expression of superoxide dismutase (SOD2), catalase (CAT), and glutathione peroxidase (GPX1) was modulated by TRF treatment, with increased activity of superoxide dismutase and catalase and reduced glutathione peroxidase in senescent myoblasts.
In comparison to ATF and NAC, TRF was more efficient in heightening the antioxidant capacity and reducing free radical insults.
These results suggested that TRF is able to ameliorate antioxidant defense mechanisms and improves replicative senescence-associated oxidative stress in myoblasts.
American Psychological Association (APA)
Khor, Shy Cian& Ngah, Wan Zurinah Wan& Mohd Yusof, Yasmin Anum& Abdul Karim, Norwahidah& Makpol, Suzana. 2017. Tocotrienol-Rich Fraction Ameliorates Antioxidant Defense Mechanisms and Improves Replicative Senescence-Associated Oxidative Stress in Human Myoblasts. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-17.
https://search.emarefa.net/detail/BIM-1194465
Modern Language Association (MLA)
Khor, Shy Cian…[et al.]. Tocotrienol-Rich Fraction Ameliorates Antioxidant Defense Mechanisms and Improves Replicative Senescence-Associated Oxidative Stress in Human Myoblasts. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-17.
https://search.emarefa.net/detail/BIM-1194465
American Medical Association (AMA)
Khor, Shy Cian& Ngah, Wan Zurinah Wan& Mohd Yusof, Yasmin Anum& Abdul Karim, Norwahidah& Makpol, Suzana. Tocotrienol-Rich Fraction Ameliorates Antioxidant Defense Mechanisms and Improves Replicative Senescence-Associated Oxidative Stress in Human Myoblasts. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-17.
https://search.emarefa.net/detail/BIM-1194465
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194465